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rdf:type |
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lifeskim:mentions |
umls-concept:C0009015,
umls-concept:C0014442,
umls-concept:C0017337,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0348801,
umls-concept:C0441836,
umls-concept:C0679058,
umls-concept:C1412099,
umls-concept:C1547699,
umls-concept:C2700640
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pubmed:issue |
12
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pubmed:dateCreated |
2003-11-7
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pubmed:databankReference |
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pubmed:abstractText |
A genomic DNA fragment corresponding to exon 7 of the human ABO gene was amplified from rats of several inbred and outbred strains. Five different sequences were obtained, four of them corresponding to A-type sequences and one to a B-type sequence based on the amino acids equivalent to residues at positions 266 and 268 of the human enzymes. In rats from inbred strains, a single A-type sequence and the unique B-type sequence were found, whereas some animals of outbred strains presented two or three A-type sequences along with the B-type sequence. The complete coding sequence of the B-type gene was obtained; identification of the exon-intron boundaries, determined by comparison with rat genomic sequences from data banks, revealed that the rat B-type gene structure is identical with that of the mouse Abo gene. Compared with the human ABO gene and the rat A gene, it lacks exon 4. Like the rat A gene (symbol: Abo), the rat B gene (symbol: Abo2) is located on chromosome 3q11-q12. It could be shown by transfection experiments that the B-type cDNA encodes an active B transferase. A transcript of the B gene was found ubiquitously, whereas the B antigen was only detected in a restricted set of tissues. These data indicate that rats have at least two distinct Abo genes, one monomorphic gene encoding a B-specific enzyme and one or more genes in some cases encoding an A-specific enzyme.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ABO Blood-Group System,
http://linkedlifedata.com/resource/pubmed/chemical/Abo protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Galactosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/N-Acetylgalactosaminyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/UDPgalactosamine-galactose...,
http://linkedlifedata.com/resource/pubmed/chemical/blood-group-substance...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0959-6658
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
919-28
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12799344-ABO Blood-Group System,
pubmed-meshheading:12799344-Amino Acid Sequence,
pubmed-meshheading:12799344-Animals,
pubmed-meshheading:12799344-CHO Cells,
pubmed-meshheading:12799344-COS Cells,
pubmed-meshheading:12799344-Cloning, Molecular,
pubmed-meshheading:12799344-Cricetinae,
pubmed-meshheading:12799344-DNA, Complementary,
pubmed-meshheading:12799344-Exons,
pubmed-meshheading:12799344-Galactosyltransferases,
pubmed-meshheading:12799344-Humans,
pubmed-meshheading:12799344-Introns,
pubmed-meshheading:12799344-Mice,
pubmed-meshheading:12799344-Molecular Sequence Data,
pubmed-meshheading:12799344-N-Acetylgalactosaminyltransferases,
pubmed-meshheading:12799344-RNA, Messenger,
pubmed-meshheading:12799344-Rats,
pubmed-meshheading:12799344-Rats, Inbred Strains,
pubmed-meshheading:12799344-Rats, Sprague-Dawley,
pubmed-meshheading:12799344-Rats, Wistar,
pubmed-meshheading:12799344-Sequence Analysis, DNA,
pubmed-meshheading:12799344-Sequence Homology, Amino Acid
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pubmed:year |
2003
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pubmed:articleTitle |
Cloning of a rat gene encoding the histo-blood group B enzyme: rats have more than one Abo gene.
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pubmed:affiliation |
INSERM U419, Institut de Biologie, 9 Quai Moncousu, 44093, Nantes Cedex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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