Linked Life Data

12798507

Source:http://linkedlifedata.com/resource/pubmed/id/12798507

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-6-11
pubmed:databankReference
pubmed:abstractText
Angiogenesis is an important step in the development of ocular onchocercaisis. In previous studies, it has been demonstrated that Onchocerca volvulus homologues of the Ancylostoma secreted protein family have pronounced angiogenic activity. The overall goal of the current study was to determine if this angiogenic effect is exerted through a direct or indirect mechanism. These studies focused on one member of this family, OvASP-2, as this protein is expressed in microfilaria, the stage of the parasite that causes ocular onchocercaisis. Clones encoding truncated and full length open reading frames were expressed as fusion proteins with Escherichia coli maltose binding protein (MBP), and angiogenic activity was compared in vitro and in vivo with MBP alone. Truncated constructs expressing only the first 105 amino acids of OvASP-2 were as active as the full length protein in inducing new blood vessel formation. The full length fusion protein did not stimulate proliferation or production of vascular endothelial growth factor in vascular endothelial cells in vitro, indicating that OvASP-2 does not directly stimulate angiogenesis. Sequence analysis demonstrated that the gene encoding OvASP-2 contained five introns. Sequence comparisons of the genomic loci from West African blinding and non-blinding strains of O. volvulus revealed that some polymorphism existed among the various isolates tested. However, none of these polymorphisms could be used to differentiate the parasite strains, suggesting that qualitative variation in OvASP-2 could not explain the difference in ocular pathogenic potential of the two parasite strains.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
61-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12798507-Ancylostoma, pubmed-meshheading:12798507-Angiogenesis Inducing Agents, pubmed-meshheading:12798507-Animals, pubmed-meshheading:12798507-Base Sequence, pubmed-meshheading:12798507-Cattle, pubmed-meshheading:12798507-Cells, Cultured, pubmed-meshheading:12798507-Cloning, Molecular, pubmed-meshheading:12798507-Cornea, pubmed-meshheading:12798507-Endothelial Growth Factors, pubmed-meshheading:12798507-Endothelium, pubmed-meshheading:12798507-Helminth Proteins, pubmed-meshheading:12798507-Humans, pubmed-meshheading:12798507-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:12798507-Lymphokines, pubmed-meshheading:12798507-Mice, pubmed-meshheading:12798507-Mice, Inbred BALB C, pubmed-meshheading:12798507-Molecular Sequence Data, pubmed-meshheading:12798507-Neovascularization, Physiologic, pubmed-meshheading:12798507-Onchocerca volvulus, pubmed-meshheading:12798507-Polymorphism, Genetic, pubmed-meshheading:12798507-Sequence Alignment, pubmed-meshheading:12798507-Vascular Endothelial Growth Factor A, pubmed-meshheading:12798507-Vascular Endothelial Growth Factors
pubmed:year
2003
pubmed:articleTitle
Angiogenic activity of an Onchocerca volvulus Ancylostoma secreted protein homologue.
pubmed:affiliation
Division of Geographic Medicine, University of Alabama at Birmingham, BBRB 203, 1530 3rd Avenue South, Birmingham, AL 35294-2170, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't