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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1992-12-11
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pubmed:databankReference |
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pubmed:abstractText |
The tetradecapeptide somatotropin-release inhibiting factor somatostatin-14 regulates the release of peptide hormones and also functions as neurotransmitter. The octacosapeptide somatostatin-28, the N-terminally extended form of somatostatin-14, shows similar biological activities yet with different potencies. Both peptides most likely function through distinct receptors. Here we report on the molecular and functional characterization of a somatostatin-28 receptor (SSR-28) cloned from a rat brain cDNA library. The nucleotide sequence contains an open reading frame for a protein of 428 amino acid residues with a predicted molecular mass of 47 kDa. Binding assays using radiolabeled somatostatin-14 and membranes from COS cells transfected with the cloned cDNA show that this receptor, SSR-28, has a higher binding affinity for somatostatin-28 (IC50 = 0.24 nM) than for somatostatin-14 (IC50 = 0.89 nM). RNA blot analysis reveals a 4.4-kilobase mRNA in rat cerebellum and at significantly lower abundance in other brain regions. In situ hybridization indicates that SSR-28 mRNA is present in the granular and Purkinje cell layers of the cerebellum and in the large cells of the hypoglossal nucleus of the brain stem. Signals for SSR-28 mRNA do not overlap with those of a previously cloned rat receptor that preferentially binds somatostatin-14 (SSR-14). SSR-14 mRNA is found in the medial cerebellar nucleus, horizontal limb of the diagonal band, various hypothalamic nuclei, and in layers IV and V of the cortex. In the rat cerebellum, SSR-14 and SSR-28 mRNAs are developmentally regulated; the levels of the former are highest around birth and levels of the latter are highest at the adult stage.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-1346068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-15499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-1652922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-1661599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-1672510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-1678852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-2014230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-2238044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-2541503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-26879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-2837314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-2864986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-2871018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-2886933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-2992313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-3803580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-4213073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-4682131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-6116516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-6117799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279674-643079
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Poly A,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Somatostatin,
http://linkedlifedata.com/resource/pubmed/chemical/Somatostatin,
http://linkedlifedata.com/resource/pubmed/chemical/Somatostatin-28
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10267-71
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1279674-Amino Acid Sequence,
pubmed-meshheading:1279674-Animals,
pubmed-meshheading:1279674-Brain,
pubmed-meshheading:1279674-Cloning, Molecular,
pubmed-meshheading:1279674-DNA,
pubmed-meshheading:1279674-Gene Expression,
pubmed-meshheading:1279674-In Situ Hybridization,
pubmed-meshheading:1279674-Kinetics,
pubmed-meshheading:1279674-Male,
pubmed-meshheading:1279674-Molecular Sequence Data,
pubmed-meshheading:1279674-Poly A,
pubmed-meshheading:1279674-RNA,
pubmed-meshheading:1279674-RNA, Antisense,
pubmed-meshheading:1279674-RNA, Messenger,
pubmed-meshheading:1279674-Rats,
pubmed-meshheading:1279674-Rats, Wistar,
pubmed-meshheading:1279674-Receptors, Somatostatin,
pubmed-meshheading:1279674-Sequence Homology, Amino Acid,
pubmed-meshheading:1279674-Somatostatin,
pubmed-meshheading:1279674-Somatostatin-28,
pubmed-meshheading:1279674-Transfection
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pubmed:year |
1992
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pubmed:articleTitle |
Molecular cloning of a somatostatin-28 receptor and comparison of its expression pattern with that of a somatostatin-14 receptor in rat brain.
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pubmed:affiliation |
Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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