Linked Life Data

12795377

Source:http://linkedlifedata.com/resource/pubmed/id/12795377

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-6-10
pubmed:abstractText
A proteomic examination of Sinorhizobium meliloti strain 1021 was undertaken using a combination of 2-D gel electrophoresis, peptide mass fingerprinting, and bioinformatics. Our goal was to identify (i) putative symbiosis- or nutrient-stress-specific proteins, (ii) the biochemical pathways active under different conditions, (iii) potential new genes, and (iv) the extent of posttranslational modifications of S. meliloti proteins. In total, we identified the protein products of 810 genes (13.1% of the genome's coding capacity). The 810 genes generated 1,180 gene products, with chromosomal genes accounting for 78% of the gene products identified (18.8% of the chromosome's coding capacity). The activity of 53 metabolic pathways was inferred from bioinformatic analysis of proteins with assigned Enzyme Commission numbers. Of the remaining proteins that did not encode enzymes, ABC-type transporters composed 12.7% and regulatory proteins 3.4% of the total. Proteins with up to seven transmembrane domains were identified in membrane preparations. A total of 27 putative nodule-specific proteins and 35 nutrient-stress-specific proteins were identified and used as a basis to define genes and describe processes occurring in S. meliloti cells in nodules and under stress. Several nodule proteins from the plant host were present in the nodule bacteria preparations. We also identified seven potentially novel proteins not predicted from the DNA sequence. Post-translational modifications such as N-terminal processing could be inferred from the data. The posttranslational addition of UMP to the key regulator of nitrogen metabolism, PII, was demonstrated. This work demonstrates the utility of combining mass spectrometry with protein arraying or separation techniques to identify candidate genes involved in important biological processes and niche occupations that may be intransigent to other methods of gene expression profiling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0894-0282
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
508-24
pubmed:meshHeading
pubmed-meshheading:12795377-Adaptation, Physiological, pubmed-meshheading:12795377-Amino Acid Sequence, pubmed-meshheading:12795377-Bacterial Proteins, pubmed-meshheading:12795377-Carbon, pubmed-meshheading:12795377-Carrier Proteins, pubmed-meshheading:12795377-Cell Surface Extensions, pubmed-meshheading:12795377-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:12795377-Endopeptidases, pubmed-meshheading:12795377-Gene Expression Profiling, pubmed-meshheading:12795377-Isoelectric Point, pubmed-meshheading:12795377-Molecular Sequence Data, pubmed-meshheading:12795377-Nitrogenase, pubmed-meshheading:12795377-Phosphorus, pubmed-meshheading:12795377-Protein Isoforms, pubmed-meshheading:12795377-Protein Processing, Post-Translational, pubmed-meshheading:12795377-Sequence Homology, Amino Acid, pubmed-meshheading:12795377-Sinorhizobium meliloti, pubmed-meshheading:12795377-Symbiosis
pubmed:year
2003
pubmed:articleTitle
A global analysis of protein expression profiles in Sinorhizobium meliloti: discovery of new genes for nodule occupancy and stress adaptation.
pubmed:affiliation
Genomic Interactions Group, Research School of Biological Sciences, Australian National University, GPO Box 475, Canberra, ACT 2601 Australia. Michael@rsbs.anu.edu.au
pubmed:publicationType
Journal Article