pubmed-article:1279381 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C0035587 | lld:lifeskim |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C0025252 | lld:lifeskim |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C1167331 | lld:lifeskim |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C0009219 | lld:lifeskim |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
pubmed-article:1279381 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
pubmed-article:1279381 | pubmed:issue | 4 | lld:pubmed |
pubmed-article:1279381 | pubmed:dateCreated | 1992-12-2 | lld:pubmed |
pubmed-article:1279381 | pubmed:abstractText | Rickettsia prowazekii (virulent Breinl strain) random genomic DNA fragments were cloned in the lambda gt11 expression vector by using non-palindromic adaptors. Several immunoreactive clones were selected after screening 20,000 individual recombinant plaques with human convalescent serum. Some recombinants synthesized the complete 60 K protein, and others synthesized beta-galactosidase fusion polypeptides containing epitopes of 134 K protein of the R. prowazekii outer membrane. The amplified genomic library was screened with monospecific antibodies directed against abundant 31 K and 29.5 K outer membrane proteins. Several recombinant clones expressing full or part of 29.5 K polypeptide, and none expressing 31 K polypeptide were revealed. The serum of a patient convalescing from epidemic typhus did not react in western blot with recombinant 29.5 K protein. | lld:pubmed |
pubmed-article:1279381 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1279381 | pubmed:language | rus | lld:pubmed |
pubmed-article:1279381 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1279381 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:1279381 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1279381 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1279381 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1279381 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1279381 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1279381 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1279381 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1279381 | pubmed:issn | 0026-8984 | lld:pubmed |
pubmed-article:1279381 | pubmed:author | pubmed-author:BalaevaN MNM | lld:pubmed |
pubmed-article:1279381 | pubmed:author | pubmed-author:Emel'ianovV... | lld:pubmed |
pubmed-article:1279381 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1279381 | pubmed:volume | 26 | lld:pubmed |
pubmed-article:1279381 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1279381 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1279381 | pubmed:pagination | 847-58 | lld:pubmed |
pubmed-article:1279381 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:1279381 | pubmed:articleTitle | [Cloning and expression in Escherichia coli of three Rickettsia prowazekii genes, coding outer membrane proteins]. | lld:pubmed |
pubmed-article:1279381 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1279381 | pubmed:publicationType | English Abstract | lld:pubmed |