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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1992-12-2
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pubmed:abstractText |
Rickettsia prowazekii (virulent Breinl strain) random genomic DNA fragments were cloned in the lambda gt11 expression vector by using non-palindromic adaptors. Several immunoreactive clones were selected after screening 20,000 individual recombinant plaques with human convalescent serum. Some recombinants synthesized the complete 60 K protein, and others synthesized beta-galactosidase fusion polypeptides containing epitopes of 134 K protein of the R. prowazekii outer membrane. The amplified genomic library was screened with monospecific antibodies directed against abundant 31 K and 29.5 K outer membrane proteins. Several recombinant clones expressing full or part of 29.5 K polypeptide, and none expressing 31 K polypeptide were revealed. The serum of a patient convalescing from epidemic typhus did not react in western blot with recombinant 29.5 K protein.
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pubmed:commentsCorrections |
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pubmed:language |
rus
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0026-8984
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
847-58
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1279381-Antibodies, Bacterial,
pubmed-meshheading:1279381-Bacterial Outer Membrane Proteins,
pubmed-meshheading:1279381-Bacteriophage lambda,
pubmed-meshheading:1279381-Blotting, Western,
pubmed-meshheading:1279381-Cloning, Molecular,
pubmed-meshheading:1279381-DNA, Bacterial,
pubmed-meshheading:1279381-Epitopes,
pubmed-meshheading:1279381-Escherichia coli,
pubmed-meshheading:1279381-Gene Expression,
pubmed-meshheading:1279381-Genes, Bacterial,
pubmed-meshheading:1279381-Genetic Vectors,
pubmed-meshheading:1279381-Humans,
pubmed-meshheading:1279381-Recombinant Fusion Proteins,
pubmed-meshheading:1279381-Rickettsia prowazekii,
pubmed-meshheading:1279381-Typhus, Endemic Flea-Borne,
pubmed-meshheading:1279381-beta-Galactosidase
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pubmed:articleTitle |
[Cloning and expression in Escherichia coli of three Rickettsia prowazekii genes, coding outer membrane proteins].
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pubmed:publicationType |
Journal Article,
English Abstract
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