Linked Life Data

12793457

Source:http://linkedlifedata.com/resource/pubmed/id/12793457

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-6-9
pubmed:abstractText
A method has been developed to determine the false-positive effects on acetylcholinesterase inhibition in the TLC assay based on Ellman's method. Various aldehydes and amines have been tested in order to determine whether the observed inhibition is due to a true enzyme inhibition or due to the inhibition of the reaction between thiocholine and 5,5'-dithiobis-(2-nitrobenzoic acid). 4-Dimethylaminobenzaldehyde, 3-ethoxy-4-hydroxybenzaldehyde, diethylamine, triethylamine, triethanolamine and tyramine showed real enzyme inhibition, although their activity was about 10(3) times lower than that shown by galanthamine. Heptanal, decanal, cinnamaldehyde, anisaldehyde, benzaldehyde, hexylamine and tryptamine appeared to show a non-specific chemical inhibition. By checking this chemical inhibition on the TLC assay, the true enzyme inhibition could be distinguished from the false-positive chemical inhibition observed in the toluene extract of Nerine bowdenii in the course of isolation of active compounds.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0958-0344
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
127-31
pubmed:meshHeading
pubmed:articleTitle
Qualitative determination of false-positive effects in the acetylcholinesterase assay using thin layer chromatography.
pubmed:affiliation
Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, PO Box 9502, 2300 RA, Leiden, The Netherlands.
pubmed:publicationType
Journal Article