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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-6-6
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pubmed:abstractText |
We analyzed the epitopes and the molecular forms of Tat recognized by the antibodies raised by Tat-toxoid vaccination in both healthy and HIV-infected volunteers. Tat-toxoid-vaccinated healthy volunteer sera reacted predominantly with peptides covering amino acids 1 through 24 and 46 through 60, corresponding to the N-terminus and basic domains of Tat. In contrast, whereas all sera from vaccinated HIV-1-positive patients reacted with the N-terminus and (with a single exception) with the basic domain, most of these sera also recognized peptides encompassing distinct domains of Tat, particularly the C-terminus (79-86). The sera of vaccinated individuals recognized both monomeric and oligomeric forms of Tat 1 through 86 or of Tat 1 through 101 and also blocked the ability of cell-released extracellular Tat to transactivate the HIV-1 LTR promoter. Synthetic Tat preincubated with sera from vaccinated individuals lost its functional activity as well. This is probably because of its inability to enter the cells as a result of immune complex formation with anti-Tat IgG. These data demonstrate that Tat-toxoid vaccination induces an efficient antibody response blocking the functional activity of Tat.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AIDS Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Immunodominant Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Inactivated,
http://linkedlifedata.com/resource/pubmed/chemical/human immunodeficiency virus type...,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1525-4135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
47-55
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12792355-AIDS Vaccines,
pubmed-meshheading:12792355-Adult,
pubmed-meshheading:12792355-Amino Acid Sequence,
pubmed-meshheading:12792355-Female,
pubmed-meshheading:12792355-Gene Products, tat,
pubmed-meshheading:12792355-HIV Antibodies,
pubmed-meshheading:12792355-HIV Infections,
pubmed-meshheading:12792355-HIV-1,
pubmed-meshheading:12792355-Humans,
pubmed-meshheading:12792355-Immune Sera,
pubmed-meshheading:12792355-Immunodominant Epitopes,
pubmed-meshheading:12792355-Male,
pubmed-meshheading:12792355-Middle Aged,
pubmed-meshheading:12792355-Molecular Sequence Data,
pubmed-meshheading:12792355-Promoter Regions, Genetic,
pubmed-meshheading:12792355-Transcriptional Activation,
pubmed-meshheading:12792355-Vaccines, Inactivated,
pubmed-meshheading:12792355-Voluntary Workers,
pubmed-meshheading:12792355-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2003
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pubmed:articleTitle |
Identification of immunodominant epitopes in inactivated Tat-vaccinated healthy and HIV-1-infected volunteers.
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pubmed:affiliation |
Tumor Progression Section, double dagger Immuno-Pharmacology Section, and #Molecular Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
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pubmed:publicationType |
Journal Article,
Clinical Trial,
Research Support, Non-U.S. Gov't
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