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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2003-9-23
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pubmed:abstractText |
Apoptosis or programmed cell death plays an important role in a wide variety of physiologic processes and is regulated by proteins of the Bcl-2 family consisting of both antiapoptotic and proapoptotic factors. The direct involvement of the Bcl-2 protein family in the process of mast cell apoptosis has not been clarified. In the present work we have used a single-chain antibody (scFv) raised against Bcl-2 derived from a semisynthetic human phage-display antibody library. The addition of TAT sequence, which is responsible for translocation through the membrane, endows the anti-Bcl-2-scFv with the ability to penetrate living cells. Moreover, it specifically neutralizes Bcl-2 intracellularly by binding to the BH1 domain and eradicates its antiapoptotic activity in 2 types of mast cells and in a human breast cancer cell line.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2506-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12791661-Amino Acid Sequence,
pubmed-meshheading:12791661-Animals,
pubmed-meshheading:12791661-Antibodies,
pubmed-meshheading:12791661-Antibody Specificity,
pubmed-meshheading:12791661-Apoptosis,
pubmed-meshheading:12791661-Breast Neoplasms,
pubmed-meshheading:12791661-Cell Survival,
pubmed-meshheading:12791661-Cloning, Molecular,
pubmed-meshheading:12791661-Gene Products, tat,
pubmed-meshheading:12791661-Humans,
pubmed-meshheading:12791661-Leukemia, Basophilic, Acute,
pubmed-meshheading:12791661-Mast Cells,
pubmed-meshheading:12791661-Membrane Potentials,
pubmed-meshheading:12791661-Mitochondria,
pubmed-meshheading:12791661-Molecular Sequence Data,
pubmed-meshheading:12791661-Peptide Library,
pubmed-meshheading:12791661-Protein Structure, Tertiary,
pubmed-meshheading:12791661-Proto-Oncogene Proteins,
pubmed-meshheading:12791661-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:12791661-Rats,
pubmed-meshheading:12791661-Tumor Cells, Cultured,
pubmed-meshheading:12791661-bcl-2-Associated X Protein
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pubmed:year |
2003
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pubmed:articleTitle |
A novel strategy using single-chain antibody to show the importance of Bcl-2 in mast cell survival.
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pubmed:affiliation |
Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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