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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1992-12-4
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pubmed:abstractText |
A newly developed antibody assay based on a synthetic peptide of 15 amino acids derived from the core region of the hepatitis C virus (HCV) genome was evaluated in serum and plasma panels of (A) 225 haemophiliacs and (B) 44 patients with chronic non-A, non-B (NANB) hepatitis, and in (C) sequential serum samples of 9 patients with transfusion transmitted HCV infection. The new anti-core peptide ELISA was compared with the anti-C100 ELISA. For confirmation of HCV infection, samples were tested in a 4-antigen recombinant immunoblot assay (4-RIBA) and samples of panels B and C were also assayed in cDNA-polymerase chain reaction (PCR). In two panels with a high prevalence of HCV infection (88.4 and 70.5% in haemophilia and NANB hepatitis patients, respectively), the sensitivity of the anti-core peptide ELISA did not differ significantly from the sensitivity of the anti-C100 ELISA. The sensitivity of the new assay as compared with the anti-C100 assay was found to be 0.84 [95% confidence interval (CI): 0.78-0.89] versus 0.92 (95% CI: 0.87-0.95) in haemophilia patients and 0.71 (95% CI: 0.52-0.86) versus 0.84 (95% CI: 0.66-0.95) in NANB hepatitis patients. In sequential serum samples of patients with transfusion-transmitted HCV infection antibodies to the core peptide (in 6/9 patients) appeared later than antibodies to C100 (in 7/9 patients): 168 (range: 70-322) and 143 (range: 59-365) days after transfusion, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/C100 protein, hepatitis C virus,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis C Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Core Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/anti-C100-3,
http://linkedlifedata.com/resource/pubmed/chemical/nucleocapsid protein, Hepatitis C...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0146-6615
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
187-91
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1279109-Amino Acid Sequence,
pubmed-meshheading:1279109-Antigens, Viral,
pubmed-meshheading:1279109-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1279109-Epitopes,
pubmed-meshheading:1279109-Evaluation Studies as Topic,
pubmed-meshheading:1279109-Hemophilia A,
pubmed-meshheading:1279109-Hepacivirus,
pubmed-meshheading:1279109-Hepatitis, Viral, Human,
pubmed-meshheading:1279109-Hepatitis Antibodies,
pubmed-meshheading:1279109-Hepatitis C,
pubmed-meshheading:1279109-Hepatitis C Antibodies,
pubmed-meshheading:1279109-Humans,
pubmed-meshheading:1279109-Molecular Sequence Data,
pubmed-meshheading:1279109-Peptides,
pubmed-meshheading:1279109-Sensitivity and Specificity,
pubmed-meshheading:1279109-Viral Core Proteins,
pubmed-meshheading:1279109-Viral Nonstructural Proteins
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pubmed:year |
1992
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pubmed:articleTitle |
Sensitivity of an anti-HCV core peptide ELISA.
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pubmed:affiliation |
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
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pubmed:publicationType |
Journal Article
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