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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-6-6
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pubmed:abstractText |
Human myeloma proteins (HMPs) from 10 patients with multiple myeloma (MM) were used to affinity-select peptides from a random phage-display peptide library. Binding peptides were identified for the 10 analysed antibodies (eight, immunoglobulin G (IgG), and two, immunoglobulin A (IgA)). The specificity of the binding was confirmed by competitive experiments using phages and chemically synthesized peptides. Interestingly, some phage-displayed peptides were immuno-selected with HMPs isolated from different patients. Sequence alignments and homology searches revealed a significant homology with human proteins (e.g. neural cell adhesion proteins) and pathogen-derived proteins (e.g. herpes simplex virus capsid proteins). The selected peptides could be useful as targeting agents for myeloma cells expressing surface immunoglobulins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0300-9475
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
583-90
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12791097-Amino Acid Sequence,
pubmed-meshheading:12791097-Antibodies, Neoplasm,
pubmed-meshheading:12791097-Antibody Specificity,
pubmed-meshheading:12791097-Binding, Competitive,
pubmed-meshheading:12791097-Humans,
pubmed-meshheading:12791097-Immunoglobulin A,
pubmed-meshheading:12791097-Immunoglobulin G,
pubmed-meshheading:12791097-Molecular Sequence Data,
pubmed-meshheading:12791097-Multiple Myeloma,
pubmed-meshheading:12791097-Myeloma Proteins,
pubmed-meshheading:12791097-Peptide Library,
pubmed-meshheading:12791097-Protein Binding,
pubmed-meshheading:12791097-Sequence Homology, Amino Acid
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pubmed:year |
2003
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pubmed:articleTitle |
Probing the specificity of human myeloma proteins with a random peptide phage library.
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pubmed:affiliation |
Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, Norway.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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