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rdf:type |
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lifeskim:mentions |
umls-concept:C0002199,
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0026764,
umls-concept:C0037083,
umls-concept:C0185117,
umls-concept:C0205182,
umls-concept:C0439855,
umls-concept:C0812265,
umls-concept:C1710082,
umls-concept:C2911684
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pubmed:issue |
23
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pubmed:dateCreated |
2003-6-5
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pubmed:abstractText |
We have previously demonstrated that the responsiveness of multiple myeloma (MM) cells to interferon-alpha (IFN-alpha) stimulation is variable, with an atypical growth response displayed by some cells. Here we report the ability of IFN-alpha to induce tyrosine phosphorylation of a 180 kDa band in the KAS-6/1 MM cell line, which is growth responsive to IFN-alpha. Further characterization demonstrated that this band corresponds to ErbB3. To our knowledge, this is the first report of ErbB3 expression in a cell type of the hematopoietic lineage. Although ErbB receptors have been shown to crosscommunicate with various other receptors, our results show for the first time that the IFN-alpha receptor can crosscommunicate with ErbB3. To address the significance of these observations, we transfected ErbB3-negative DP-6 MM cells with ErbB3 and used siRNA to silence ErbB3 in the KAS-6/1 cell line. Although IFN-alpha transactivated ErbB3 in the DP-6 transfectants, it did not confer growth responsiveness to IFN-alpha. Interestingly, silencing ErbB3 expression in the KAS-6/1 cells decreased the overall growth response to IFN-alpha and to interleukin-6. These results suggest that ErbB3 expression alone does not uniquely confer IFN-alpha growth responsiveness, but instead may amplify proliferation rates in MM cells that have acquired atypical expression of this receptor.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Interferon alpha-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interferon,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3598-607
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12789268-Cell Division,
pubmed-meshheading:12789268-DNA-Binding Proteins,
pubmed-meshheading:12789268-Down-Regulation,
pubmed-meshheading:12789268-Enzyme Activation,
pubmed-meshheading:12789268-Female,
pubmed-meshheading:12789268-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12789268-Humans,
pubmed-meshheading:12789268-Interferon-alpha,
pubmed-meshheading:12789268-Kinetics,
pubmed-meshheading:12789268-Macromolecular Substances,
pubmed-meshheading:12789268-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12789268-Multiple Myeloma,
pubmed-meshheading:12789268-Phosphorylation,
pubmed-meshheading:12789268-RNA, Small Interfering,
pubmed-meshheading:12789268-Receptor, Interferon alpha-beta,
pubmed-meshheading:12789268-Receptor, erbB-3,
pubmed-meshheading:12789268-Receptor Cross-Talk,
pubmed-meshheading:12789268-Receptors, Interferon,
pubmed-meshheading:12789268-STAT3 Transcription Factor,
pubmed-meshheading:12789268-Signal Transduction,
pubmed-meshheading:12789268-Trans-Activators,
pubmed-meshheading:12789268-Tumor Cells, Cultured,
pubmed-meshheading:12789268-Tyrosine
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pubmed:year |
2003
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pubmed:articleTitle |
Atypical expression of ErbB3 in myeloma cells: cross-talk between ErbB3 and the interferon-alpha signaling complex.
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pubmed:affiliation |
Department of Immunology, Tumor Biology Program, Mayo Graduate and Medical Schools, Mayo Clinic/Foundation, Rochester, MN 55905, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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