Linked Life Data

12788512

Source:http://linkedlifedata.com/resource/pubmed/id/12788512

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-6-5
pubmed:abstractText
N-methyl-D-aspartate (NMDA) may affect dopaminergic cells, which contain tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine synthesis. To clarify the involvement of TH in the neuroprotective effects of nitric oxide (NO), we investigated whether NMDA alters TH mRNA and TH protein levels and whether NO inhibits NMDA-induced changes in the rat retina. Dopamine levels in the retina were measured by high-performance liquid chromatography (HPLC). Reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR showed that intravitreal injection of NMDA caused a significant reduction in TH mRNA levels in the retina. Similarly, Western blot analysis showed that NMDA decreased the production of TH protein. These reductions in TH mRNA and TH protein levels were attenuated by concomitant injection of NOC 18, an NO donor. HPLC analysis showed that NMDA reduced dopamine levels in the retina and that NO attenuated this reduction. Furthermore, morphological analysis showed that NO prevents NMDA-induced neurotoxicity through dopamine D(1) receptors. These results suggest that the neuroprotective effect of NO may be associated with the induction of TH expression and increased levels of dopamine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
977
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
46-54
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12788512-Animals, pubmed-meshheading:12788512-Benzazepines, pubmed-meshheading:12788512-Blotting, Western, pubmed-meshheading:12788512-Cell Count, pubmed-meshheading:12788512-Chromatography, High Pressure Liquid, pubmed-meshheading:12788512-Domperidone, pubmed-meshheading:12788512-Dopamine, pubmed-meshheading:12788512-Dopamine Antagonists, pubmed-meshheading:12788512-Drug Interactions, pubmed-meshheading:12788512-Excitatory Amino Acid Agonists, pubmed-meshheading:12788512-Free Radical Scavengers, pubmed-meshheading:12788512-Gene Expression Regulation, pubmed-meshheading:12788512-Male, pubmed-meshheading:12788512-N-Methylaspartate, pubmed-meshheading:12788512-Neurotoxicity Syndromes, pubmed-meshheading:12788512-Nitric Oxide, pubmed-meshheading:12788512-Nitric Oxide Donors, pubmed-meshheading:12788512-Nitroso Compounds, pubmed-meshheading:12788512-Rats, pubmed-meshheading:12788512-Rats, Wistar, pubmed-meshheading:12788512-Retina, pubmed-meshheading:12788512-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12788512-Time Factors, pubmed-meshheading:12788512-Tyrosine 3-Monooxygenase
pubmed:year
2003
pubmed:articleTitle
Neuroprotective effect of nitric oxide against NMDA-induced neurotoxicity in the rat retina is associated with tyrosine hydroxylase expression.
pubmed:affiliation
Department of Ophthalmology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki-shi, Kanagawa 216-8511, Japan. kitaoka@marianna-u.ac.jp
pubmed:publicationType
Journal Article, Comparative Study