Source:http://linkedlifedata.com/resource/pubmed/id/12787001
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2003-6-5
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| pubmed:abstractText |
A combination of specific HLA class II antigens and the presence of type 1 diabetes (T1D)-related antibodies has a high positive predictive value for T1D but low sensitivity. The aim of the present study was to determine the frequencies of HLA-DRB-DQB deduced haplotypes associated with susceptibility and protection in Slovenian patients with established T1D, to evaluate the relationship between the HLA-DRB1-QBP-DQB1 haplotypes and the presence of insulin autoantibodies (IAA) and glutamic acid decarboxylase antibodies (GADA), and to access the possible impact of polymorphic QBP promoters on this relationship. A cohort of 135 patients with T1D (age 17.5 +/- 7.0 years, duration of T1D 9.14 +/- 6.3 years) was investigated. HLA-DRB1 and DQB1 alleles were typed using the polymerase chain reaction (PCR)-reverse line blot method. QBP promoter region alleles were determined using PCR-sequence-specific oligonucleotide hybridization (SSO) and PCR-sequence-specific primers (SSP). IAA and GADA antibodies were determined by enzyme-linked immunosorbent assay (ELISA). The chi-square test with Yates' correction was used for statistical analysis. Deduced haplotypes DRB1*0301-DQB1*0201 (P = 0.0001, OR = 3.4), DRB1*0401-DQB1*0302 (P = 0.0001, OR = 29.8), and DRB1*0402-DQB1*0302 (P = 0.008, OR = 4.7) were significantly more common, and DRB1*1501-DQB1*0602 (P = 0.0001, OR = 0.03) significantly less common in the investigated cohort than in a Slovenian control group. The highest risk and the strongest protective HLA-DR-DQ haplotypes found in Slovenian patients with T1D did not differ from those found in other Caucasian populations. While the DRB1*0301-QBP2.1-DQB1*0201 haplotype, where QBP2.1 did not help to further distinguish DQB1*0201-possessing haplotypes in IAA-positive and IAA-negative patients, was strongly associated with the presence of IAA, the DRB1*0101-QBP5.12-DQB1*0501 haplotype, although not protective compared to the control population, was associated with an absence of IAA in the investigated cohort. It is suggested that there may be a combined influence of the QBP5.12 promoter and the DQB1*0501 functional molecule on reduced IAA production.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Decarboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DQ Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DQ beta-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DQB1 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DRB1*03:01 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DRB1*04:01 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DRB1*04:02 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DRB1 Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0960-7420
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
30
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
223-7
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12787001-Adolescent,
pubmed-meshheading:12787001-Adult,
pubmed-meshheading:12787001-Antibodies,
pubmed-meshheading:12787001-Autoantibodies,
pubmed-meshheading:12787001-Case-Control Studies,
pubmed-meshheading:12787001-Child,
pubmed-meshheading:12787001-Child, Preschool,
pubmed-meshheading:12787001-Diabetes Mellitus, Type 1,
pubmed-meshheading:12787001-European Continental Ancestry Group,
pubmed-meshheading:12787001-Gene Frequency,
pubmed-meshheading:12787001-Genetic Predisposition to Disease,
pubmed-meshheading:12787001-Glutamate Decarboxylase,
pubmed-meshheading:12787001-HLA-DQ Antigens,
pubmed-meshheading:12787001-HLA-DQ beta-Chains,
pubmed-meshheading:12787001-HLA-DR Antigens,
pubmed-meshheading:12787001-HLA-DRB1 Chains,
pubmed-meshheading:12787001-Humans,
pubmed-meshheading:12787001-Insulin,
pubmed-meshheading:12787001-Polymorphism, Genetic,
pubmed-meshheading:12787001-Slovenia
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| pubmed:year |
2003
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| pubmed:articleTitle |
The HLA-DRB, -DQB polymorphism and anti-insulin antibody response in Slovenian patients with type 1 diabetes.
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| pubmed:affiliation |
University Children's Hospital, Department of Pediatric Endocrinology, Diabetes and Metabolic Diseases, Ljubljana, Slovenia. tadej.battelino@mf.uni-lj.si
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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