Linked Life Data

12783195

Source:http://linkedlifedata.com/resource/pubmed/id/12783195

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-6-3
pubmed:abstractText
Two pyruvate decarboxylase genes, pdcA, and pdcB, were cloned from Rhizopus oryzae. These genes are similar to each other with approximately 85% nucleotide sequence identity within the coding region. Multiple transcriptional start sites and polyadenylation sites were found for both genes. The deduced translation product of each gene results in a 561 amino acid protein with approximate molecular weight of 61 kDa each. The amino acid identity between the two proteins was 91% as calculated by Lipmann-Pearson comparisons. Transcriptional control appears to be important in regulation of the PDC, since much of the transcript accumulation parallels enzymatic activity. There was no detectable pdc transcript from cultures grown in glycerol-containing medium. Induction of transcription for pdcA and pdcB was initiated within 1.5 h of adding glucose to the culture. Shifting the aerobically grown cultures to anoxic conditions at this time resulted in enhanced pdc transcription, PDC enzymatic activity, and ethanol production, compared to cultures with continued aerobic growth.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0343-8651
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-64
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Induction of Rhizopus oryzae pyruvate decarboxylase genes.
pubmed:affiliation
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 1815 N University Street, Peoria, IL 61604, USA. skorycd@ncaur.usda.gov
pubmed:publicationType
Journal Article