Source:http://linkedlifedata.com/resource/pubmed/id/12782599
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
2003-6-3
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pubmed:abstractText |
The CCL6 chemokine gene was identified as a direct positive target of the L-Myc oncoprotein in interleukin 3-dependent 32D myeloid cells. A mutant form of c-Myc, lacking a region of the NH(2)-terminal domain necessary for transcriptional repression (c-MycDeltaMBII), also up-regulated CCL6. Chromatin immunoprecipitation showed that L-Myc, c-MycDeltaMBII, and full-length c-Myc all bound the CCL6 promoter, although the latter was inactive in transcriptional up-regulation. Exogenously added CCL6 induced marked apoptosis in some cell types. However, in 32D cells, the coexpression of c-Myc and CCL6 abrogated interleukin 3 dependence and produced a highly leukemogenic phenotype. In two solid tumor models, CCL6 overexpression also accelerated tumor growth, and/or enhanced local and metastatic spread in association with marked apoptosis of the tumor capsule and adjacent normal tissues. Our results show that CCL6 can be either a positive or negative target for Myc oncoproteins. The chemokine may alter tumor behavior by relieving its growth factor dependency and by promoting invasiveness as a result of local tissue apoptosis.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0008-5472
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2923-32
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12782599-Animals,
pubmed-meshheading:12782599-Apoptosis,
pubmed-meshheading:12782599-Carcinoma, Squamous Cell,
pubmed-meshheading:12782599-Cell Division,
pubmed-meshheading:12782599-Cell Survival,
pubmed-meshheading:12782599-Chemokines, CC,
pubmed-meshheading:12782599-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12782599-HeLa Cells,
pubmed-meshheading:12782599-Humans,
pubmed-meshheading:12782599-Mice,
pubmed-meshheading:12782599-Neoplasm Metastasis,
pubmed-meshheading:12782599-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:12782599-Transcription, Genetic,
pubmed-meshheading:12782599-Transfection,
pubmed-meshheading:12782599-Up-Regulation
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pubmed:year |
2003
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pubmed:articleTitle |
The CCL6 chemokine is differentially regulated by c-Myc and L-Myc, and promotes tumorigenesis and metastasis.
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pubmed:affiliation |
Department of Pediatrics, Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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