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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
2003-6-3
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pubmed:abstractText |
The synthesis of proliferating cell nuclear antigen (PCNA) is strictly regulated during the cell cycle. To investigate the contribution of the promoter region to the up-regulation of human PCNA expression at the onset of S phase, we have examined 17 putative elements with reporter assays in quiescent L-O2 cells and following serum stimulation. The E2F-like sequence 5'-TTCCCCGCAA-3' located at -84 to -75 is required for the serum-induced transactivation. In electrophoretic mobility shift assays, nuclear extracts from asynchronous L-O2 cells exhibit two binding activities toward the -75 E2F oligonucleotide, and the minor band, whose formation could be interfered with by E2F-1 antibody, represents an S phase-specific complex. This is the first demonstration of the E2F site in the human PCNA 5' promoter as a serum-responsive element.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
544
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
112-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12782299-Cell Cycle Proteins,
pubmed-meshheading:12782299-Cell Division,
pubmed-meshheading:12782299-Cell Line,
pubmed-meshheading:12782299-Cell Nucleus,
pubmed-meshheading:12782299-Cell Separation,
pubmed-meshheading:12782299-Cloning, Molecular,
pubmed-meshheading:12782299-DNA-Binding Proteins,
pubmed-meshheading:12782299-E2F Transcription Factors,
pubmed-meshheading:12782299-E2F1 Transcription Factor,
pubmed-meshheading:12782299-Flow Cytometry,
pubmed-meshheading:12782299-G0 Phase,
pubmed-meshheading:12782299-G1 Phase,
pubmed-meshheading:12782299-Humans,
pubmed-meshheading:12782299-Luciferases,
pubmed-meshheading:12782299-Plasmids,
pubmed-meshheading:12782299-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:12782299-Promoter Regions, Genetic,
pubmed-meshheading:12782299-Protein Binding,
pubmed-meshheading:12782299-Time Factors,
pubmed-meshheading:12782299-Transcription Factors,
pubmed-meshheading:12782299-Transfection,
pubmed-meshheading:12782299-Up-Regulation
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pubmed:year |
2003
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pubmed:articleTitle |
An E2F site in the 5'-promoter region contributes to serum-dependent up-regulation of the human proliferating cell nuclear antigen gene.
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pubmed:affiliation |
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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