Source:http://linkedlifedata.com/resource/pubmed/id/12781984
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2003-6-3
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pubmed:abstractText |
We explored the possibility that the hormone 3,3',5-tri-iodothyronine can regulate the biosynthesis of the mitochondrial calcium uniporter. To meet this objective experiments on Ca(2+) transport, and binding of the specific inhibitor Ru(360) were carried out in mitochondria isolated from euthyroid, hyperthyroid and hypothyroid rats. It was found that V(max) for Ca(2+) transport increased from 11.67+/-0.8 in euthyroid to 14.36+/-0.44 in hyperthyroid, and decreased in hypothyroid mitochondria to 8.62+/-0.63 nmol Ca(2+)/mg/s. Furthermore, the K(i) for the specific inhibitor Ru(360), depends on the thyroid status, i.e. 18, 19 and 13 nM for control, hyper- and hypothyroid mitochondria, respectively. In addition, the binding of 103Ru(360) was increased in hyperthyroid and decreased in hypothyroid mitochondria. Scatchard analysis for the binding of 103Ru(360) showed the following values: 28, 40 and 23 pmol/mg for control, hyper- and hypothyroid mitochondria, respectively. The K(d) for 103Ru(360) was found to be 30.39, 37.03 and 35.71 nM for controls, hyper- and hypothyroid groups, respectively. When hypothyroid rats were treated with thyroid hormone, mitochondrial Ca(2+) transport, as well as 103Ru(360) binding, reached similar values to those found for euthyroid mitochondria.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ru 360,
http://linkedlifedata.com/resource/pubmed/chemical/Ruthenium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Triiodothyronine,
http://linkedlifedata.com/resource/pubmed/chemical/mitochondrial calcium uniporter
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1096-4959
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
135
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-82
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12781984-Animals,
pubmed-meshheading:12781984-Biological Transport,
pubmed-meshheading:12781984-Calcium,
pubmed-meshheading:12781984-Calcium Channels,
pubmed-meshheading:12781984-Calcium-Binding Proteins,
pubmed-meshheading:12781984-Energy Metabolism,
pubmed-meshheading:12781984-Hyperthyroidism,
pubmed-meshheading:12781984-Hypothyroidism,
pubmed-meshheading:12781984-Mitochondria, Liver,
pubmed-meshheading:12781984-Rats,
pubmed-meshheading:12781984-Ruthenium Compounds,
pubmed-meshheading:12781984-Triiodothyronine
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pubmed:year |
2003
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pubmed:articleTitle |
Thyroid hormone may induce changes in the concentration of the mitochondrial calcium uniporter.
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pubmed:affiliation |
Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, Juan Badiano # 1 Tlalpan, México, D.F. 014080, Mexico.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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