Linked Life Data

12781698

Source:http://linkedlifedata.com/resource/pubmed/id/12781698

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-6-3
pubmed:abstractText
Echinacea purpurea, a plant originally used by native Americans to treat respiratory infections, has also been shown to exert immunomodulatory activities both in vivo and in vitro. However, the mechanism underlying Echinacea-induced immunomodulation remains largely unknown. This study examined in vitro the effects of soluble extracts of E. purpurea on natural killer (NK) cells present in human peripheral blood mononuclear cells (PBMC). Flow cytometric methods were used to examine activation, cytotoxicity, NK-target binding, and killer cell frequency. Treatment of PBMC with Echinacea overnight resulted in the activation of CD69 expression and increase in mean fluorescence intensity in both the CD16+ and CD16+CD56+ NK subsets. However, the frequency of CD16+ cells was decreased as well as the mean fluorescence intensity was down-regulated. NK cytotoxicity was augmented 100% at the concentration of 0.1 microg/ml of Echinacea in a short time (4-h) assay. Examination at the single cell level revealed augmentation of the frequency of CD56+ NK-target conjugates and a plateau was reached after 30-60 min of incubation. Likewise, the frequency of CD56+ killer cells in the conjugates was also significantly increased by Echinacea. There was recruitment of non-conjugated CD56+ cells into CD16+ NK-target conjugates and activation of the NK-target non-killer conjugates into killer cells. These findings demonstrate that Echinacea extracts are potent activators of NK cytotoxicity. Echinacea augments the frequency of NK target conjugates and activates the programming for lysis of NK cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1567-5769
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
811-24
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:12781698-Antigens, CD, pubmed-meshheading:12781698-Antigens, CD56, pubmed-meshheading:12781698-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:12781698-Antigens, Surface, pubmed-meshheading:12781698-Cell Line, pubmed-meshheading:12781698-Echinacea, pubmed-meshheading:12781698-Flow Cytometry, pubmed-meshheading:12781698-Green Fluorescent Proteins, pubmed-meshheading:12781698-Humans, pubmed-meshheading:12781698-Killer Cells, Natural, pubmed-meshheading:12781698-Lectins, C-Type, pubmed-meshheading:12781698-Luminescent Proteins, pubmed-meshheading:12781698-Lymphocytes, pubmed-meshheading:12781698-Macrophage Activation, pubmed-meshheading:12781698-Monocytes, pubmed-meshheading:12781698-Plant Extracts, pubmed-meshheading:12781698-Receptors, IgG, pubmed-meshheading:12781698-T-Lymphocytes, Regulatory
pubmed:year
2003
pubmed:articleTitle
Mechanism of activation of human peripheral blood NK cells at the single cell level by Echinacea water soluble extracts: recruitment of lymphocyte-target conjugates and killer cells and activation of programming for lysis.
pubmed:affiliation
Department of Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, 10833 Le Conte Avenue, A2-060 CHS, Los Angeles, CA 90095-1747, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.