Linked Life Data

12781684

Source:http://linkedlifedata.com/resource/pubmed/id/12781684

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-6-3
pubmed:abstractText
The transcription factor E74 is one of the early genes induced by ecdysteroids during metamorphosis of Drosophila melanogaster. Here, we report the cloning and hormonal regulation of E74 from the tobacco hornworm, Manduca sexta (MsE74). MsE74 is 98% identical to that of D. melanogaster within the DNA-binding ETS domain of the protein. The 5'-isoform-specific regions of MsE74A and MsE74B share significantly lower sequence similarity (30-40%). Developmental expression by Northern blot analysis reveals that, during the 5th larval instar, MsE74B expression correlates with pupal commitment on day 3 and is induced to maximal levels within 12h by low levels of 20-hydroxyecdysone (20E) and repressed by physiologically relevant levels of juvenile hormone I (JH I). Immunocytochemical analysis shows that MsE74B appears in the epidermis before the 20E-induced Broad transcription factor that is correlated with pupal commitment (Zhou and Riddiford, 2001). In contrast, MsE74A is expressed late in the larval and the pupal molts when the ecdysteroid titer has declined to low levels and in the adult molt just as the ecdysteroid titer begins to decline. This change in timing during the adult molt appears not to be due to the absence of JH as there was no change during the pupal molt of allatectomized animals. When either 4th or 5th instar larval epidermis was explanted and subjected to hormonal manipulations, MsE74A induction occurred only after exposure to 20E followed by its removal. Thus, MsE74B appears to have a similar role at the onset of metamorphosis in Manduca as it does in Drosophila, whereas MsE74A is regulated differently at pupation in Manduca than at pupariation in Drosophila.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
76-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12781684-Amino Acid Sequence, pubmed-meshheading:12781684-Animals, pubmed-meshheading:12781684-Base Sequence, pubmed-meshheading:12781684-Cells, Cultured, pubmed-meshheading:12781684-Drosophila, pubmed-meshheading:12781684-Ecdysterone, pubmed-meshheading:12781684-Epidermis, pubmed-meshheading:12781684-Gene Expression Regulation, Developmental, pubmed-meshheading:12781684-Genes, Insect, pubmed-meshheading:12781684-Insect Proteins, pubmed-meshheading:12781684-Juvenile Hormones, pubmed-meshheading:12781684-Larva, pubmed-meshheading:12781684-Manduca, pubmed-meshheading:12781684-Metamorphosis, Biological, pubmed-meshheading:12781684-Molecular Sequence Data, pubmed-meshheading:12781684-Open Reading Frames, pubmed-meshheading:12781684-Protein Isoforms, pubmed-meshheading:12781684-Protein Structure, Tertiary, pubmed-meshheading:12781684-Pupa, pubmed-meshheading:12781684-RNA, Messenger, pubmed-meshheading:12781684-Sequence Homology, Amino Acid, pubmed-meshheading:12781684-Transcription Factors
pubmed:year
2003
pubmed:articleTitle
E74 exhibits stage-specific hormonal regulation in the epidermis of the tobacco hornworm, manduca sexta.
pubmed:affiliation
Department of Biology, University of Washington, Box 351800, Seattle, WA 98195-1800, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.