Linked Life Data

1278150

Source:http://linkedlifedata.com/resource/pubmed/id/1278150

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-8-23
pubmed:abstractText
Uteroglobin, an hormonally induced protein composed of two similar subunits, represents around 50% of the proteins synthesized and secreted into the uterine lumen of rabbits treated sequentially with estradiol and progesterone. The endometrium of these animals was used as a source for the isolation of the mRNA for uteroglobin. Poly(A)-rich RNA, extracted from purified polysomes with phenol chloroform and isolated on oligo(dT)-cellulose columns, contains one fourth of the total protein coding activity of the endometrium. Between 20--25% of the polypeptides synthesized by this RNA in cell-free systems derived from Krebs II ascites cells or wheat germs react with a monospecific antiserum prepared in guinea pigs against uteroglobin. The material bound to the antibody was identified as a precursor of uteroglobin according to the following criteria. 1. The product synthesized in vitro can be displaced from the complex with the specific immunoglobulin by purified uteroglobin. 2. Analysis of the immunoprecipitate on polyacrylamide gels containing urea and dodecylsulfate demonstrate the existence of a single labelled polypeptide with an apparent molecular weight larger than the uteroglobin subunits. 3. The tryptic digest of this polypeptide, labelled in vitro with [3H]lysine, shares seven peptides with mature uteroglobin labelled with the same amino acid in perfused uteri, and exhibits and additional peptide not present in uteroglobin. 4. Injection of the same mRNA preparation into Xenopus oocytes results in the production of uteroglobin. The endometrium of intact animals treated with estradiol alone also contains the same mRNA bound to polysomes but in a smaller proportion, indicating that the progesterone-induced synthesis of uteroglobin is accompanied by an accumulation of the specific mRNA in the polysomes.
pubmed:keyword
http://linkedlifedata.com/resource/pubmed/keyword/Animals, Laboratory, http://linkedlifedata.com/resource/pubmed/keyword/Antibodies--analysis, http://linkedlifedata.com/resource/pubmed/keyword/Biology, http://linkedlifedata.com/resource/pubmed/keyword/Clinical Research, http://linkedlifedata.com/resource/pubmed/keyword/ESTRADIOL, http://linkedlifedata.com/resource/pubmed/keyword/Endocrine System, http://linkedlifedata.com/resource/pubmed/keyword/Estrogens, http://linkedlifedata.com/resource/pubmed/keyword/Genitalia, http://linkedlifedata.com/resource/pubmed/keyword/Genitalia, Female, http://linkedlifedata.com/resource/pubmed/keyword/Hematological Effects, http://linkedlifedata.com/resource/pubmed/keyword/Hemic System, http://linkedlifedata.com/resource/pubmed/keyword/Hormones, http://linkedlifedata.com/resource/pubmed/keyword/IMMUNITY, http://linkedlifedata.com/resource/pubmed/keyword/Immunoglobulin Alterations, http://linkedlifedata.com/resource/pubmed/keyword/Immunologic Factors, http://linkedlifedata.com/resource/pubmed/keyword/In Vitro, http://linkedlifedata.com/resource/pubmed/keyword/PROGESTERONE, http://linkedlifedata.com/resource/pubmed/keyword/Physiology, http://linkedlifedata.com/resource/pubmed/keyword/Progestational Hormones, http://linkedlifedata.com/resource/pubmed/keyword/Research Methodology, http://linkedlifedata.com/resource/pubmed/keyword/Urogenital System, http://linkedlifedata.com/resource/pubmed/keyword/Uterus
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-25
pubmed:dateRevised
2007-7-23
pubmed:otherAbstract
PIP: Evidence for the translocation of messenger ribonucleic acid (mRNA) for rabbit uteroglobin in cell-free systems is presented. Endometrium of rabbits was used as a source for the isolation of the mRNA for uteroglobin. Poly(A)-rich RNA extracted from purified polysomes with phenol chloroform and isolated on oligo(DT)-cellulose columns contained 1/4 of the total protein coding activity of the endometrium. 20-25% of the polypeptides synthesized by this RNA in cell-free systems derived from Krebs 2 ascites cells or wheat germs reacted with a monospecific antiserum prepared in guinea pigs against uteroglobin. The following criteria were used to identify the material bound to the antibody as a precursor of uteroglobin: 1) the product synthesized in vitro could be displaced from the complex with the specific immunoglobulin by purified uteroglobin; 2) analysis of the immunoprecipitate on polyacrylamide gels containing urea and dodecylsulfate revealed the existence of a single labeled polypeptide with an apparent molecular weight larger than the uteroglobin subunits; 3) the tryptic digest of this polypeptide, labeled in vitro with tritiated lysine shares 7 peptides with mature uteroglobin labeled with the same amino acid in perfused uteri and exhibited an additional peptide not present in uteroglobin; and 4) injection of the same mRNA preparation into Xenopus oocytes resulted in the production of uteroglobin. Endometrium of intact animals treated with estradiol alone also contained the same mRNA bound to polysomes but in a smaller proportion, indicating that progesterone-induced synthesis of uteroglobin is accompanied by an accumulation of the specific mRNA in the polysomes.
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Translation of the mRNA for rabbit uteroglobin in cell-free systems. Evidence for a precursor protein.
pubmed:publicationType
Journal Article