Linked Life Data

12777385

Source:http://linkedlifedata.com/resource/pubmed/id/12777385

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
36
pubmed:dateCreated
2003-9-1
pubmed:abstractText
Nop56p is a component of the box C/D small nucleolar ribonucleoprotein complexes that direct 2'-O-methylation of pre-rRNA during its maturation. Genetic analyses in yeast have shown that Nop56p plays important roles in the early steps of pre-rRNA processing. However, its precise function remains elusive, especially in higher eukaryotes. Here we describe the proteomic characterization of human Nop56p (hNop56p)-associated pre-ribosomal ribonucleoprotein complexes. Mass spectrometric analysis of purified pre-ribosomal ribonucleoprotein complexes identified 61 ribosomal proteins, 16 trans-acting factors probably involved in ribosome biogenesis, and 29 proteins whose function in ribosome biogenesis is unknown. Identification of pre-rRNA species within hNop56p-associated pre-ribosomal ribonucleoprotein complexes, coupled with the known functions of yeast orthologs of the probable trans-acting factors identified in human, demonstrated that hNop56p functions in the early to middle stages of 60 S subunit synthesis in human cells. Interestingly, the nucleolar phosphoprotein treacle, which is responsible for the craniofacial disorder associated with Treacher Collins syndrome, was found to be a constituent of hNop56p-associated pre-rRNP complexes. The association of hNop56p and treacle within the complexes was independent of rRNA integrity, indicating a direct interaction. In addition, the protein compositions of the treacle-associated and hNop56p-associated pre-ribosomal ribonucleoprotein complexes were very similar, suggesting functional similarities between these two complexes with respect to ribosome biogenesis in human cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
278
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
34309-19
pubmed:dateRevised
2011-10-14
pubmed:meshHeading
pubmed-meshheading:12777385-Amino Acid Motifs, pubmed-meshheading:12777385-Blotting, Northern, pubmed-meshheading:12777385-Cell Line, pubmed-meshheading:12777385-Cell Nucleolus, pubmed-meshheading:12777385-Cell Nucleus, pubmed-meshheading:12777385-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:12777385-Epitopes, pubmed-meshheading:12777385-Humans, pubmed-meshheading:12777385-Immunohistochemistry, pubmed-meshheading:12777385-Mandibulofacial Dysostosis, pubmed-meshheading:12777385-Mass Spectrometry, pubmed-meshheading:12777385-Models, Genetic, pubmed-meshheading:12777385-Nuclear Proteins, pubmed-meshheading:12777385-Phosphoproteins, pubmed-meshheading:12777385-Precipitin Tests, pubmed-meshheading:12777385-Protein Binding, pubmed-meshheading:12777385-Protein Structure, Tertiary, pubmed-meshheading:12777385-Proteome, pubmed-meshheading:12777385-RNA, Ribosomal, pubmed-meshheading:12777385-Ribonucleases, pubmed-meshheading:12777385-Ribonucleoproteins, pubmed-meshheading:12777385-Ribosomes, pubmed-meshheading:12777385-Spectrometry, Mass, Matrix-Assisted Laser..., pubmed-meshheading:12777385-Transfection
pubmed:year
2003
pubmed:articleTitle
Proteomic analysis of human Nop56p-associated pre-ribosomal ribonucleoprotein complexes. Possible link between Nop56p and the nucleolar protein treacle responsible for Treacher Collins syndrome.
pubmed:affiliation
Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't