Source:http://linkedlifedata.com/resource/pubmed/id/12774179
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2003-8-4
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pubmed:databankReference | |
pubmed:abstractText |
A full-length cDNA encoding CCAAT/enhancer-binding protein beta (C/EBPbeta) was cloned from rainbow trout by anchored PCR. The putative 291 amino acid protein has 53% and 32% identity to the zebrafish and Japanese flounder sequences, respectively, and 30-34% identity to tetrapod homologues. This clone contains most conserved C/EBPbeta domains except the second transactivation domain just like the zebrafish homologue. Also similar to zebrafish, rainbow trout produces only shorter C/EBPbeta isoforms (LAP and LIP) but not the longer isoform (LAP*). However, unlike the zebrafish and Japanese flounder homologues, trout C/EBPbeta has the short open reading frame (uORF) upstream of the start codon for LAP but in an alternate reading frame, a feature of tetrapod C/EBPbeta genes. In normal rainbow trout, C/EBPbeta mRNA was detected in peripheral blood leukocytes, head kidney, posterior kidney, liver, spleen, gills, intestine and muscle. RT-PCR revealed that transcript levels of trout C/EBPbeta are clearly higher in sodium alginate-induced peritoneal cells than in head kidney and peritoneal cells of saline-injected fish or head kidney cells of alginate-injected fish. Together with expression in immunologically important tissues, this indicates that C/EBPbeta is likely to be involved in the immune response just as in mammals. Southern hybridization suggested that C/EBPbeta is a single copy gene. There are no introns in this C/EBPbeta gene, just like the mammalian homologues. These data suggest that we have obtained the trout ortholog of C/EBPbeta.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0093-7711
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
253-61
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12774179-Amino Acid Sequence,
pubmed-meshheading:12774179-Animals,
pubmed-meshheading:12774179-Base Sequence,
pubmed-meshheading:12774179-CCAAT-Enhancer-Binding Protein-beta,
pubmed-meshheading:12774179-Cloning, Molecular,
pubmed-meshheading:12774179-DNA, Complementary,
pubmed-meshheading:12774179-Evolution, Molecular,
pubmed-meshheading:12774179-Gene Dosage,
pubmed-meshheading:12774179-Molecular Sequence Data,
pubmed-meshheading:12774179-Oncorhynchus mykiss,
pubmed-meshheading:12774179-Phylogeny,
pubmed-meshheading:12774179-RNA, Messenger,
pubmed-meshheading:12774179-Sequence Homology, Amino Acid,
pubmed-meshheading:12774179-Tissue Distribution
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pubmed:year |
2003
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pubmed:articleTitle |
Molecular cloning and characterization of rainbow trout (Oncorhynchus mykiss) CCAAT/enhancer binding protein beta.
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pubmed:affiliation |
Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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