Source:http://linkedlifedata.com/resource/pubmed/id/12774123
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6939
|
| pubmed:dateCreated |
2003-5-29
|
| pubmed:databankReference | |
| pubmed:abstractText |
Embryonic stem (ES) cells are pluripotent cells derived from early mammalian embryos. Their immortality and rapid growth make them attractive sources for stem cell therapies; however, they produce tumours (teratomas) when transplanted, which could preclude their therapeutic usage. Why ES cells, which lack chromosomal abnormalities, possess tumour-like properties is largely unknown. Here we show that mouse ES cells specifically express a Ras-like gene, which we have named ERas. We show that human HRasp, which is a recognized pseudogene, does not contain reported base substitutions and instead encodes the human orthologue of ERas. This protein contains amino-acid residues identical to those present in active mutants of Ras and causes oncogenic transformation in NIH 3T3 cells. ERas interacts with phosphatidylinositol-3-OH kinase but not with Raf. ERas-null ES cells maintain pluripotency but show significantly reduced growth and tumorigenicity, which are rescued by expression of ERas complementary DNA or by activated phosphatidylinositol-3-OH kinase. We conclude that the transforming oncogene ERas is important in the tumour-like growth properties of ES cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Protein p21(ras),
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0028-0836
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
29
|
| pubmed:volume |
423
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
541-5
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:12774123-3T3 Cells,
pubmed-meshheading:12774123-Amino Acid Sequence,
pubmed-meshheading:12774123-Animals,
pubmed-meshheading:12774123-Cell Division,
pubmed-meshheading:12774123-Cell Transformation, Neoplastic,
pubmed-meshheading:12774123-Cloning, Molecular,
pubmed-meshheading:12774123-Embryo, Mammalian,
pubmed-meshheading:12774123-Genes, ras,
pubmed-meshheading:12774123-Guanosine Diphosphate,
pubmed-meshheading:12774123-Guanosine Triphosphate,
pubmed-meshheading:12774123-Mice,
pubmed-meshheading:12774123-Molecular Sequence Data,
pubmed-meshheading:12774123-Neoplasms,
pubmed-meshheading:12774123-Oncogene Protein p21(ras),
pubmed-meshheading:12774123-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:12774123-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:12774123-Pseudogenes,
pubmed-meshheading:12774123-Stem Cells
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Role of ERas in promoting tumour-like properties in mouse embryonic stem cells.
|
| pubmed:affiliation |
Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
|
| pubmed:publicationType |
Journal Article
|