Linked Life Data

12774122

Source:http://linkedlifedata.com/resource/pubmed/id/12774122

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6939
pubmed:dateCreated
2003-5-29
pubmed:abstractText
Rhomboid proteins are intramembrane serine proteases that activate epidermal growth factor receptor (EGFR) signalling in Drosophila. Rhomboids are conserved throughout evolution, and even in eukaryotes their existence in species with no EGFRs implies that they must have additional roles. Here we report that Saccharomyces cerevisiae has two rhomboids, which we have named Rbd1p and Rbd2p. RBD1 deletion results in a respiratory defect; consistent with this, Rbd1p is localized in the inner mitochondrial membrane and mutant cells have disrupted mitochondria. We have identified two substrates of Rbd1p: cytochrome c peroxidase (Ccp1p); and a dynamin-like GTPase (Mgm1p), which is involved in mitochondrial membrane fusion. Rbd1p mutants are indistinguishable from Mgm1p mutants, indicating that Mgm1p is a key substrate of Rbd1p and explaining the rbd1Delta mitochondrial phenotype. Our data indicate that mitochondrial membrane remodelling is regulated by cleavage of Mgm1p and show that intramembrane proteolysis by rhomboids controls cellular processes other than signalling. In addition, mitochondrial rhomboids are conserved throughout eukaryotes and the mammalian homologue, PARL, rescues the yeast mutant, suggesting that these proteins represent a functionally conserved subclass of rhomboid proteases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
423
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
537-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12774122-Amino Acid Sequence, pubmed-meshheading:12774122-Cell Division, pubmed-meshheading:12774122-Cell Respiration, pubmed-meshheading:12774122-Conserved Sequence, pubmed-meshheading:12774122-Cytochrome-c Peroxidase, pubmed-meshheading:12774122-GTP-Binding Proteins, pubmed-meshheading:12774122-Gene Deletion, pubmed-meshheading:12774122-Intracellular Membranes, pubmed-meshheading:12774122-Mitochondria, pubmed-meshheading:12774122-Mitochondrial Proteins, pubmed-meshheading:12774122-Molecular Sequence Data, pubmed-meshheading:12774122-Phenotype, pubmed-meshheading:12774122-Protein Processing, Post-Translational, pubmed-meshheading:12774122-Saccharomyces cerevisiae, pubmed-meshheading:12774122-Saccharomyces cerevisiae Proteins, pubmed-meshheading:12774122-Serine Endopeptidases
pubmed:year
2003
pubmed:articleTitle
Mitochondrial membrane remodelling regulated by a conserved rhomboid protease.
pubmed:affiliation
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't