Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-9-22
pubmed:abstractText
Analysis by cDNA microarrays showed that in the murine epididymis, NaPi-IIb was the predominantly expressed epithelial isoform of the sodium-inorganic phosphate cotransporter and was markedly overexpressed in the proximal region in the infertile knockout (KO) compared to the fertile heterozygous (HET) c-ros transgenic mouse. The apparent up-regulation in the KO mouse confirmed by Northern and Western blot analyses could be explained by the absence of NaPi-IIb from the initial segment of the HET epididymis, as revealed by immunohistochemistry, and its presence on the epithelial brush border throughout the proximal epididymis of KO mice, where differentiation of the initial segment fails to occur. Both NaPi-IIb mRNA and protein were scarce or absent from the cauda epididymidis of both genotypes. A high content of inorganic phosphate was measured enzymatically in the HET cauda luminal fluid, with a 27% decrease in the KO mice. This decrease, presumably from a greater reabsorption of inorganic phosphate, particularly in the initial part of the KO epididymis, may disturb the normal process of sperm maturation in these infertile males. By contrast, no apparent consequences were observed for the transport of Na+ and Ca2+, the concentrations of which (approximately 26 mM and approximately 30 microM, respectively) were measured by microelectrodes to be identical in the caudal fluid from both genotypes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1135-41
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12773415-Animals, pubmed-meshheading:12773415-Body Fluids, pubmed-meshheading:12773415-Calcium, pubmed-meshheading:12773415-Epididymis, pubmed-meshheading:12773415-Female, pubmed-meshheading:12773415-Fertility, pubmed-meshheading:12773415-Infertility, Male, pubmed-meshheading:12773415-Male, pubmed-meshheading:12773415-Mice, pubmed-meshheading:12773415-Mice, Knockout, pubmed-meshheading:12773415-Mice, Transgenic, pubmed-meshheading:12773415-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:12773415-Phosphates, pubmed-meshheading:12773415-Proto-Oncogene Proteins, pubmed-meshheading:12773415-RNA, pubmed-meshheading:12773415-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:12773415-Sodium, pubmed-meshheading:12773415-Sodium-Phosphate Cotransporter Proteins, pubmed-meshheading:12773415-Sodium-Phosphate Cotransporter Proteins, Type IIb, pubmed-meshheading:12773415-Spermatozoa, pubmed-meshheading:12773415-Symporters
pubmed:year
2003
pubmed:articleTitle
Sodium-inorganic phosphate cotransporter NaPi-IIb in the epididymis and its potential role in male fertility studied in a transgenic mouse model.
pubmed:affiliation
Institute of Reproductive Medicine of the University, Münster, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't