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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-5-29
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pubmed:abstractText |
Hedgehog signaling is considered to play a crucial role in chondrogenesis by regulation through a network of cytokine actions, which is not fully understood. We examined the effect of hedgehog signaling on the expression of core-binding factor a1 (Cbfa1), a critical transcription factor for the development of bone and cartilage. Primary chondrocytes prepared from the costal cartilage of newborn mice were treated with N-terminal fragment of recombinant murine sonic hedgehog (rmShh-N). Northern blot analysis indicated that Cbfa1 mRNA expression levels in the chondrocyte cultures were elevated by the treatment with rmShh-N. rmShh-N treatment enhanced 1.8 kb Cbfa1 promoter activity in chondrocytes, suggesting the presence of transcriptional control. As Cbfa1-binding site(s) have been located in the promoter of the receptor activator of nuclear factor-kappaB (RANK) ligand (RANKL) gene, we also examined RANKL expression. rmShh-N treatment upregulated RANKL and RANK mRNA expression levels in chondrocytes. Interestingly, RANKL suppressed the hedgehog enhancement of alkaline phosphatase activity in chondrocytes, suggesting the presence of a link between these signaling molecules. We conclude that hedgehog signaling activates Cbfa1 gene expression through its promoter in chondrocytes, and also activates and interacts with RANKL to maintain cartilage development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Core Binding Factor alpha Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hedgehog Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RANK Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Activator of Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfrsf11a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfsf11 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/core binding factor alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-0795
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
413-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12773122-Analysis of Variance,
pubmed-meshheading:12773122-Animals,
pubmed-meshheading:12773122-Animals, Newborn,
pubmed-meshheading:12773122-Blotting, Northern,
pubmed-meshheading:12773122-Carrier Proteins,
pubmed-meshheading:12773122-Chondrocytes,
pubmed-meshheading:12773122-Core Binding Factor alpha Subunits,
pubmed-meshheading:12773122-DNA-Binding Proteins,
pubmed-meshheading:12773122-Gene Expression Regulation,
pubmed-meshheading:12773122-Hedgehog Proteins,
pubmed-meshheading:12773122-Luciferases,
pubmed-meshheading:12773122-Membrane Glycoproteins,
pubmed-meshheading:12773122-Mice,
pubmed-meshheading:12773122-Mice, Inbred ICR,
pubmed-meshheading:12773122-RANK Ligand,
pubmed-meshheading:12773122-RNA, Messenger,
pubmed-meshheading:12773122-Receptor Activator of Nuclear Factor-kappa B,
pubmed-meshheading:12773122-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12773122-Signal Transduction,
pubmed-meshheading:12773122-Trans-Activators,
pubmed-meshheading:12773122-Transcription Factors,
pubmed-meshheading:12773122-Transfection
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pubmed:year |
2003
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pubmed:articleTitle |
Hedgehog signaling enhances core-binding factor a1 and receptor activator of nuclear factor-kappaB ligand (RANKL) gene expression in chondrocytes.
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pubmed:affiliation |
Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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