Source:http://linkedlifedata.com/resource/pubmed/id/12771037
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2003-5-28
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| pubmed:abstractText |
Dietary omega-3 polyunsaturated fatty acids (omega-3 PUFAs) protect against photocarcinogenesis in animals, but prospective human studies are scarce. The mechanism(s) underlying the photoprotection are uncertain, although omega-3 PUFAs may influence oxidative stress. We examined the effect of supplementation on a range of indicators of ultraviolet radiation (UVR)-induced DNA damage in humans, and assessed effect on basal and post-UVR oxidative status. In a double-blind randomized study, 42 healthy subjects took 4 g daily of purified omega-3 PUFA, eicosapentaenoic acid (EPA), or monounsaturated, oleic acid (OA), for 3 months. EPA was bioavailable; the skin content at 3 months showing an 8-fold rise from baseline, P < 0.01. No consistent pattern of alteration in basal and UVR-exposed skin content of the antioxidants glutathione, vitamins E and C or lipid peroxidation, was seen on supplementation. Sunburn sensitivity was reduced on EPA, the UVR-induced erythemal threshold rising from a mean of 36 (SD 10) mJ/cm(2) at baseline to 49 (16) mJ/cm(2) after supplementation, P < 0.01. Moreover, UVR-induced skin p53 expression, assessed immunohistochemically at 24 h post-UVR exposure, fell from a mean of 16 (SD 5) positive cells/100 epidermal cells at baseline to 8 (4) after EPA supplementation, P < 0.01. Peripheral blood lymphocytes (PBL) sampled on 3 successive days both pre- and post-supplementation, showed no change with respect to basal DNA single-strand breaks or oxidative base modification (8-oxo-dG). However, when susceptibility of PBL to ex vivo UVR was examined using the comet assay, this revealed a reduction in tail moment from 84.4 (SD 3.4) at baseline to 69.4 (3.1) after EPA, P = 0.03. No significant changes were seen in any of the above parameters following OA supplementation. Reduction in this range of early markers, i.e. sunburn, UVR-induced p53 in skin and strand breaks in PBL, indicate protection by dietary EPA against acute UVR-induced genotoxicity; longer-term supplementation might reduce skin cancer in humans.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ascorbic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Eicosapentaenoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Genetic Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Oleic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/Vitamin E
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| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0143-3334
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| pubmed:author |
pubmed-author:AzurdiaRichard MRM,
pubmed-author:Beijersbergen van HenegouwenGerard M JGM,
pubmed-author:DeanMichael PMP,
pubmed-author:EpeBerndB,
pubmed-author:HomburgMarie IMI,
pubmed-author:McArdleFF,
pubmed-author:MoisonRalf M WRM,
pubmed-author:RhodesLesley ELE,
pubmed-author:ShahbakhtiHassanH,
pubmed-author:SteenwinkelMarie-Jose S TMJ,
pubmed-author:VinkArie AAA
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| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
919-25
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12771037-Adult,
pubmed-meshheading:12771037-Aged,
pubmed-meshheading:12771037-Ascorbic Acid,
pubmed-meshheading:12771037-Biological Availability,
pubmed-meshheading:12771037-DNA,
pubmed-meshheading:12771037-DNA Damage,
pubmed-meshheading:12771037-DNA Repair,
pubmed-meshheading:12771037-Dietary Supplements,
pubmed-meshheading:12771037-Double-Blind Method,
pubmed-meshheading:12771037-Eicosapentaenoic Acid,
pubmed-meshheading:12771037-Female,
pubmed-meshheading:12771037-Genetic Markers,
pubmed-meshheading:12771037-Glutathione,
pubmed-meshheading:12771037-Humans,
pubmed-meshheading:12771037-Lipid Peroxidation,
pubmed-meshheading:12771037-Lymphocytes,
pubmed-meshheading:12771037-Male,
pubmed-meshheading:12771037-Middle Aged,
pubmed-meshheading:12771037-Neoplasms, Radiation-Induced,
pubmed-meshheading:12771037-Oleic Acid,
pubmed-meshheading:12771037-Skin,
pubmed-meshheading:12771037-Skin Neoplasms,
pubmed-meshheading:12771037-Tumor Suppressor Protein p53,
pubmed-meshheading:12771037-Ultraviolet Rays,
pubmed-meshheading:12771037-Vitamin E
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| pubmed:year |
2003
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| pubmed:articleTitle |
Effect of eicosapentaenoic acid, an omega-3 polyunsaturated fatty acid, on UVR-related cancer risk in humans. An assessment of early genotoxic markers.
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| pubmed:affiliation |
Photobiology Unit, Dermatology Centre, University of Manchester, UK. lesley.e.rhodes@man.ac.uk
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| pubmed:publicationType |
Journal Article,
Clinical Trial,
Comparative Study,
Randomized Controlled Trial,
Research Support, Non-U.S. Gov't
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