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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2003-5-28
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pubmed:abstractText |
The diverse cytoplasmic domain sequences within the various integrin subunits are critical for integrin-mediated signaling into the cell (outside-in signaling) and for activation of ligand binding affinity (inside-out signaling). Here we introduce an approach based on phage display technology to identify molecules that specifically interact with the cytoplasmic domain of the beta 5 integrin subunit. We show that a peptide selected for binding specifically to the beta 5 cytoplasmic domain (VVISYSMPD) induces apoptosis upon internalization. The cell death process induced by VVISYSMPD is sensitive to modulation by growth factors and by protein kinase C (PKC), and it cannot be triggered in beta 5 null cells. Finally, we show that the VVISYSMPD peptide is a mimic of annexin V. Our results suggest a functional link between the alpha v beta 5 integrin, annexin V, and programmed cell death. We propose the term "endothanatos" to designate this phenomenon.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1151-62
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12769841-Amino Acid Sequence,
pubmed-meshheading:12769841-Annexin A5,
pubmed-meshheading:12769841-Apoptosis,
pubmed-meshheading:12769841-Cell Adhesion,
pubmed-meshheading:12769841-Cell Line,
pubmed-meshheading:12769841-Cell Membrane,
pubmed-meshheading:12769841-Eukaryotic Cells,
pubmed-meshheading:12769841-Genetic Vectors,
pubmed-meshheading:12769841-Growth Substances,
pubmed-meshheading:12769841-Humans,
pubmed-meshheading:12769841-Integrins,
pubmed-meshheading:12769841-Molecular Mimicry,
pubmed-meshheading:12769841-Molecular Sequence Data,
pubmed-meshheading:12769841-Peptides,
pubmed-meshheading:12769841-Protein Kinase C,
pubmed-meshheading:12769841-Protein Structure, Tertiary,
pubmed-meshheading:12769841-Receptors, Vitronectin,
pubmed-meshheading:12769841-Signal Transduction
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pubmed:year |
2003
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pubmed:articleTitle |
Alpha v beta 5 integrin-dependent programmed cell death triggered by a peptide mimic of annexin V.
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pubmed:affiliation |
M.D. Anderson Cancer Center, The University of Texas, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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