| pubmed-article:12767924 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12767924 | lifeskim:mentions | umls-concept:C1518565 | lld:lifeskim |
| pubmed-article:12767924 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:12767924 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:12767924 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:12767924 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12767924 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:12767924 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:12767924 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:12767924 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:12767924 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:12767924 | pubmed:dateCreated | 2003-5-27 | lld:pubmed |
| pubmed-article:12767924 | pubmed:abstractText | Odorant responsiveness of a mouse olfactory receptor, mOR-EG, was investigated in various heterologous cells using a variety of detection methods. Odorant-induced Ca(2+) response was observed in HEK293 cells that coexpressed mOR-EG and the promiscuous G protein, G alpha 15. Without G alpha 15, a robust increase in cAMP level was observed upon odorant-stimulation in various mammalian cells. A luciferase reporter gene assay using zif268 promoter was adopted to amplify the cAMP signals. In Xenopus laevis oocytes, odorant-stimulated currents were recorded when mOR-EG cRNA was co-injected with either G alpha 15 or cAMP-dependent channel. These results suggest that odorant responsiveness can be monitored via a signaling pathway mediated by endogenous G alphas or transfected G alpha 15 in heterologous cell systems. Various functional assays for a heterologously expressed olfactory receptor reported in this study, are potentially useful for high-throughput ligand screening and functional analyses of hundreds of olfactory receptors. | lld:pubmed |
| pubmed-article:12767924 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12767924 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12767924 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12767924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12767924 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12767924 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:12767924 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:12767924 | pubmed:author | pubmed-author:KataokaHirosh... | lld:pubmed |
| pubmed-article:12767924 | pubmed:author | pubmed-author:TouharaKazush... | lld:pubmed |
| pubmed-article:12767924 | pubmed:author | pubmed-author:NakagawaTakao... | lld:pubmed |
| pubmed-article:12767924 | pubmed:author | pubmed-author:KatadaSayakoS | lld:pubmed |
| pubmed-article:12767924 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12767924 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:12767924 | pubmed:volume | 305 | lld:pubmed |
| pubmed-article:12767924 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12767924 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12767924 | pubmed:pagination | 964-9 | lld:pubmed |
| pubmed-article:12767924 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:12767924 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12767924 | pubmed:articleTitle | Odorant response assays for a heterologously expressed olfactory receptor. | lld:pubmed |
| pubmed-article:12767924 | pubmed:affiliation | Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 277-8562, Chiba, Japan | lld:pubmed |
| pubmed-article:12767924 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12767924 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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