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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-5-27
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pubmed:abstractText |
Odorant responsiveness of a mouse olfactory receptor, mOR-EG, was investigated in various heterologous cells using a variety of detection methods. Odorant-induced Ca(2+) response was observed in HEK293 cells that coexpressed mOR-EG and the promiscuous G protein, G alpha 15. Without G alpha 15, a robust increase in cAMP level was observed upon odorant-stimulation in various mammalian cells. A luciferase reporter gene assay using zif268 promoter was adopted to amplify the cAMP signals. In Xenopus laevis oocytes, odorant-stimulated currents were recorded when mOR-EG cRNA was co-injected with either G alpha 15 or cAMP-dependent channel. These results suggest that odorant responsiveness can be monitored via a signaling pathway mediated by endogenous G alphas or transfected G alpha 15 in heterologous cell systems. Various functional assays for a heterologously expressed olfactory receptor reported in this study, are potentially useful for high-throughput ligand screening and functional analyses of hundreds of olfactory receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
305
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
964-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12767924-Animals,
pubmed-meshheading:12767924-CHO Cells,
pubmed-meshheading:12767924-COS Cells,
pubmed-meshheading:12767924-Calcium,
pubmed-meshheading:12767924-Cell Line,
pubmed-meshheading:12767924-Cricetinae,
pubmed-meshheading:12767924-Cyclic AMP,
pubmed-meshheading:12767924-Electric Conductivity,
pubmed-meshheading:12767924-Eugenol,
pubmed-meshheading:12767924-GTP-Binding Protein alpha Subunits, Gq-G11,
pubmed-meshheading:12767924-Genes, Reporter,
pubmed-meshheading:12767924-Heterotrimeric GTP-Binding Proteins,
pubmed-meshheading:12767924-Humans,
pubmed-meshheading:12767924-Immunoblotting,
pubmed-meshheading:12767924-Luciferases,
pubmed-meshheading:12767924-Mice,
pubmed-meshheading:12767924-Odors,
pubmed-meshheading:12767924-Oocytes,
pubmed-meshheading:12767924-PC12 Cells,
pubmed-meshheading:12767924-Patch-Clamp Techniques,
pubmed-meshheading:12767924-Rats,
pubmed-meshheading:12767924-Receptors, Odorant,
pubmed-meshheading:12767924-Response Elements,
pubmed-meshheading:12767924-Second Messenger Systems,
pubmed-meshheading:12767924-Transfection,
pubmed-meshheading:12767924-Xenopus laevis
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pubmed:year |
2003
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pubmed:articleTitle |
Odorant response assays for a heterologously expressed olfactory receptor.
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pubmed:affiliation |
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 277-8562, Chiba, Japan
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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