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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-5-27
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pubmed:abstractText |
Transplantation of islets is becoming an established method for treating type 1 diabetes. However, viability of islets is greatly affected by necrosis/apoptosis induced by oxidative stress and other insults during isolation and subsequent in vitro culture. Expression of cytoprotective proteins, such as heme oxygenase-1 (HO-1), reduces the deleterious effects of oxidative stress in transplantable islets. We have generated a fusion protein composed of HO-1 and TAT protein transduction domain (TAT/PTD), an 11-aa cell penetrating peptide from the human immunodeficiency virus TAT protein. Transduction of TAT/PTD-HO-1 to insulin-producing cells protects against TNF-alpha-mediated cytotoxicity. TAT/PTD-HO-1 transduction to islets does not impair islet physiology, as assessed by reversion of chemically induced diabetes in immunodeficient mice. Finally, we report that transduction of HO-1 fusion protein into islets improves islet viability in culture. This approach might have a positive impact on the availability of islets for transplantation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
305
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
876-81
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:12767912-Amino Acid Sequence,
pubmed-meshheading:12767912-Animals,
pubmed-meshheading:12767912-Cell Culture Techniques,
pubmed-meshheading:12767912-Cell Line,
pubmed-meshheading:12767912-Cell Survival,
pubmed-meshheading:12767912-Cells, Cultured,
pubmed-meshheading:12767912-Cytoprotection,
pubmed-meshheading:12767912-Gene Products, tat,
pubmed-meshheading:12767912-Heme Oxygenase (Decyclizing),
pubmed-meshheading:12767912-Heme Oxygenase-1,
pubmed-meshheading:12767912-Insulin,
pubmed-meshheading:12767912-Islets of Langerhans,
pubmed-meshheading:12767912-Islets of Langerhans Transplantation,
pubmed-meshheading:12767912-Peptides,
pubmed-meshheading:12767912-Protein Structure, Tertiary,
pubmed-meshheading:12767912-Protein Transport,
pubmed-meshheading:12767912-Rats,
pubmed-meshheading:12767912-Rats, Inbred Lew,
pubmed-meshheading:12767912-Recombinant Fusion Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
Heme oxygenase-1 fused to a TAT peptide transduces and protects pancreatic beta-cells.
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pubmed:affiliation |
Diabetes Research Institute, University of Miami School of Medicine, 1450 NW 10th Avenue (R-134), Miami, FL 33136, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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