Linked Life Data

12767076

Source:http://linkedlifedata.com/resource/pubmed/id/12767076

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-5-26
pubmed:abstractText
Carbonic anhydrase IX (CA IX) is frequently expressed in human carcinomas and absent from the corresponding normal tissues. Strong induction by tumor hypoxia predisposes CA IX to serve as a target for cancer diagnostics and therapy. Here we evaluated targeting properties and pharmacokinetics of CA IX-specific monoclonal antibody (MAb) M75. Binding parameters of (125)I-labeled M75, including equilibrium dissociation constant, hypoxia-related binding to various cell lines and internalization, were analyzed in vitro. Biodistribution of (125)I-M75 in nude mice bearing HT-29 human colorectal carcinoma xenografts with hypoxic pattern of CA IX expression was studied by measurements of radioactivity in dissected tissues and macroautoradiography of tissue sections. Pharmacokinetics of intravenously administered (125)I-M75 was described using a 2-compartment model. Blood clearance showed a distribution phase t(1/2)(alpha) = 3.4 hr and an elimination phase t(1/2)(beta) = 55.3 hr postinjection. Despite predominant CA IX localization in less accessible perinecrotic regions, (125)I-M75 exhibited specific accumulation in xenograft, with a mean uptake of 15.3 +/- 3.6% of injected dose per gram of tumor tissue at 48 hr postadministration. Specificity of M75 localization was confirmed by low tumor uptake of control antibody. Altogether, our data demonstrate that M75 MAb is a promising tool for selective immunotargeting of hypoxic human tumors that express CA IX.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0020-7136
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
873-81
pubmed:dateRevised
2011-8-1
pubmed:meshHeading
pubmed-meshheading:12767076-Animals, pubmed-meshheading:12767076-Antibodies, Monoclonal, pubmed-meshheading:12767076-Antibody Specificity, pubmed-meshheading:12767076-Antigens, Neoplasm, pubmed-meshheading:12767076-Autoradiography, pubmed-meshheading:12767076-Carbonic Anhydrases, pubmed-meshheading:12767076-Carcinoma, pubmed-meshheading:12767076-Cell Hypoxia, pubmed-meshheading:12767076-Colorectal Neoplasms, pubmed-meshheading:12767076-HT29 Cells, pubmed-meshheading:12767076-Humans, pubmed-meshheading:12767076-Immunohistochemistry, pubmed-meshheading:12767076-Iodine Radioisotopes, pubmed-meshheading:12767076-Kinetics, pubmed-meshheading:12767076-Mice, pubmed-meshheading:12767076-Mice, Nude, pubmed-meshheading:12767076-Neoplasm Proteins, pubmed-meshheading:12767076-Tissue Distribution, pubmed-meshheading:12767076-Tumor Cells, Cultured, pubmed-meshheading:12767076-Tumor Markers, Biological, pubmed-meshheading:12767076-Xenograft Model Antitumor Assays
pubmed:year
2003
pubmed:articleTitle
Biodistribution and pharmacokinetics of 125I-labeled monoclonal antibody M75 specific for carbonic anhydrase IX, an intrinsic marker of hypoxia, in nude mice xenografted with human colorectal carcinoma.
pubmed:affiliation
Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't