Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2003-5-26
pubmed:abstractText
Cbl-associated protein (CAP) is an adaptor protein that plays important roles in both signal transduction and cytoskeleton rearrangement. Alternative splicing of the gene SORBS1 results in multiple isoforms of CAP protein. We report here the cloning of 3 new CAP isoforms, CAP2, CAP3, and CAP4, from mouse adipose tissue. RT-PCR analyses reveal that the isoform mRNAs are differentially expressed. CAP2, CAP3, and CAP4 contain a coiled-coil domain. In addition, CAP4 contains a proline-rich region, part of which exists in CAP3. Coimmunoprecipitation experiments show that CAP4 forms a homodimeric complex. While these new isoforms similarly interact with Cbl, they exhibit varied binding specificity toward vinculin. In contrast to CAP1 and CAP2, CAP4 does not interact with vinculin, and CAP3 binds with low affinity. Immunofluorescence analysis demonstrates differential subcellular localization of Myc-tagged CAP isoforms in 3T3-L1 adipocytes. These results suggest that these new isoforms of CAP might play different signaling roles.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-10085297, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-10394363, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-10521485, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-10657980, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-10734046, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-11001060, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-11309621, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-11371513, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-11374898, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-11481476, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-11545730, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-11557983, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-11911877, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-11997497, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-12185851, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-1339701, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-193837, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-2031185, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-8918191, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-8986783, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-9211900, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-9447983, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-9461600, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-9630982, http://linkedlifedata.com/resource/pubmed/commentcorrection/12765336-9885244
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1076-1551
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18-25
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:12765336-3T3 Cells, pubmed-meshheading:12765336-Adipocytes, pubmed-meshheading:12765336-Alternative Splicing, pubmed-meshheading:12765336-Amino Acid Sequence, pubmed-meshheading:12765336-Animals, pubmed-meshheading:12765336-COS Cells, pubmed-meshheading:12765336-Cercopithecus aethiops, pubmed-meshheading:12765336-Cloning, Molecular, pubmed-meshheading:12765336-Cytoskeletal Proteins, pubmed-meshheading:12765336-DNA Primers, pubmed-meshheading:12765336-Dimerization, pubmed-meshheading:12765336-Fluorescent Antibody Technique, pubmed-meshheading:12765336-Mice, pubmed-meshheading:12765336-Molecular Sequence Data, pubmed-meshheading:12765336-Protein Isoforms, pubmed-meshheading:12765336-RNA, Messenger, pubmed-meshheading:12765336-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12765336-Sequence Homology, Amino Acid, pubmed-meshheading:12765336-Signal Transduction, pubmed-meshheading:12765336-Subcellular Fractions, pubmed-meshheading:12765336-Vinculin
pubmed:articleTitle
Cloning and characterization of Cbl-associated protein splicing isoforms.
pubmed:affiliation
Department of Internal Medicine and Physiology, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.