rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-8-13
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pubmed:abstractText |
Activation of myocardial kappa-opioid receptor-protein kinase C (PKC) pathways may improve postischemic contractile function through a myofilament reduction in ATP utilization. To test this, we first examined the effects of PKC inhibitors on kappa-opioid receptor-dependent cardioprotection. The kappa-opioid receptor agonist U50,488H (U50) increased postischemic left ventricular developed pressure and reduced postischemic end-diastolic pressure compared with controls. PKC inhibitors abolished the cardioprotective effects of U50. To determine whether kappa-opioid-PKC-dependent decreases in Ca2+-dependent actomyosin Mg2+-ATPase could account for cardioprotection, we subjected hearts to three separate actomyosin ATPase-lowering protocols. We observed that moderate decreases in myofibrillar ATPase were equally cardioprotective as kappa-opioid receptor stimulation. Immunoblot analysis and confocal microscopy revealed a kappa-opioid-induced increase in myofilament-associated PKC-epsilon, and myofibrillar Ca2+-independent PKC activity was increased after kappa-opioid stimulation. This PKC-myofilament association led to an increase in troponin I and C-protein phosphorylation. Thus we propose PKC-epsilon activation and translocation to the myofilaments causes a decrease in actomyosin ATPase, which contributes to the kappa-opioid receptor-dependent cardioprotective mechanism.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3,4-Dichloro-N-methyl-N-(2-(1-pyrrol...,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Analgesics, Non-Narcotic,
http://linkedlifedata.com/resource/pubmed/chemical/Cardiotonic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Prkce protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-epsilon,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, kappa
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0363-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
285
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H1220-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:12763745-3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-ben...,
pubmed-meshheading:12763745-Actin Cytoskeleton,
pubmed-meshheading:12763745-Adenosine Triphosphatases,
pubmed-meshheading:12763745-Adenosine Triphosphate,
pubmed-meshheading:12763745-Analgesics, Non-Narcotic,
pubmed-meshheading:12763745-Animals,
pubmed-meshheading:12763745-Cardiotonic Agents,
pubmed-meshheading:12763745-Female,
pubmed-meshheading:12763745-Ischemic Preconditioning, Myocardial,
pubmed-meshheading:12763745-Isoenzymes,
pubmed-meshheading:12763745-Myocardial Ischemia,
pubmed-meshheading:12763745-Phosphorylation,
pubmed-meshheading:12763745-Protein Kinase C,
pubmed-meshheading:12763745-Protein Kinase C-epsilon,
pubmed-meshheading:12763745-Rats,
pubmed-meshheading:12763745-Rats, Wistar,
pubmed-meshheading:12763745-Receptors, Opioid, kappa,
pubmed-meshheading:12763745-Ventricular Pressure
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pubmed:year |
2003
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pubmed:articleTitle |
Cardioprotection through a PKC-dependent decrease in myofilament ATPase.
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pubmed:affiliation |
Department of Physiology, University of Tennessee-Memphis, 894 Union Avenue, Memphis, TN 38163, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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