Linked Life Data

12763583

Source:http://linkedlifedata.com/resource/pubmed/id/12763583

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-5-23
pubmed:abstractText
Trout POMC-A exhibits a unique C-terminal extension of 25-amino acids which is processed in the pituitary and hypothalamus to generate two novel decapeptides, EQWGREEGEE and ALGERKYHFQ-NH(2). The fibers containing these two decapeptides are widely distributed in the brain, suggesting that they may exert neurotransmitter or neuromodulator activities. In the present study, we have investigated the ontogeny of the decapeptide EQWGREEGEE in the trout pituitary and brain. In the pituitary of 29-day embryos and 33-day alevins, EQWGREEGEE-immunoreactive material was observed in a cluster of cells located in the central and rostral region of the gland, respectively. In 47-day alevins, a second group of cells exhibiting EQWGREEGEE-like immunoreactivity was detected in the caudal region of the pituitary and the intensity of labeling in these cells increased in 61-day fry. In the brain, EQWGREEGEE immunoreactivity was detected in 47-day alevins. In 47- and 61-day larvae, immunoreactive elements were mainly detected in the diencephalon. Characterization of the immunoreactive material by reversed-phase high-performance liquid chromatographic analysis combined with radioimmunoassay detection revealed the existence of two major forms which exhibited different retention times than synthetic EQWGREEGEE. The present study indicates that EQWGREEGEE-related peptides are present in the trout pituitary early during ontogeny and appear in the brain only later, and that processing of the C-terminal extension of POMC-A generates distinct molecular species at different developmental stages. These data suggest that alternative processing of the C-terminal domain of POMC-A gives rise to various peptide products that may exert specific activities during trout development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0165-3806
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-97
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Ontogeny of a novel decapeptide derived from POMC-A in the brain and pituitary of the rainbow trout.
pubmed:affiliation
European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont-Saint-Aignan, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't