Linked Life Data

12762840

Source:http://linkedlifedata.com/resource/pubmed/id/12762840

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2003-5-23
pubmed:abstractText
Availability of the complete sequence of the human genome and sequence homology analysis has accelerated new protein discovery and clues to protein function. Protein-protein interaction cloning suggests multisubunit complexes and pathways. Here, we combine these molecular approaches with cultured cell colocalization analysis to suggest a novel complex and a pathway that integrate the mitochondrial location and the microtubular cytoskeleton with chromosome remodeling, apoptosis, and tumor suppression based on a novel leucine-rich pentatricopeptide repeat-motif-containing protein (LRPPRC) that copurified with the fibroblast growth factor receptor complex. One round of interaction cloning and sequence homology analysis defined a primary LRPPRC complex with novel subunits cat eye syndrome chromosome region candidate 2 (CECR2), ubiquitously expressed transcript (UXT), and chromosome 19 open reading frames 5 (C19ORF5) but still of unknown function. Immuno, deoxyribonucleic acid (DNA), and green fluorescent protein (GFP) tag colocalization analyses revealed that LRPPRC appears in both cytosol and nuclei of cultured cells, colocalizes with mitochondria and beta-tubulin rather than with alpha-actin in the cytosol of interphase cells, and exhibits phase-dependent organization around separating chromosomes in mitotic cells. GFP-tagged CECR2B was strictly nuclear and colocalized with condensed DNA in apoptotic cells. GFP-tagged UXT and GFP-tagged C19ORF5 appeared in both cytosol and nuclei and colocalized with LRPPRC and beta-tubulin. Cells exhibiting nuclear C19ORF5 were apoptotic. Screening for interactive substrates with the primary LRPPRC substrates in the human liver complementary DNA library revealed that CECR2B interacted with chromatin-associated TFIID-associated protein TAFII30 and ribonucleic acid splicing factor SRP40, UXT bridged to CBP/p300-binding factor CITED2 and kinetochore-associated factor BUB3, and C19ORF5 complexed with mitochondria-associated NADH dehydrogenase I and cytochrome c oxidase I. C19ORF5 also interacted with RASSF1, providing a bridge to apoptosis and tumor suppression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1071-2690
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
582-94
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12762840-Carcinoma, Hepatocellular, pubmed-meshheading:12762840-Cell Culture Techniques, pubmed-meshheading:12762840-Chromosome Mapping, pubmed-meshheading:12762840-Chromosomes, Human, Pair 19, pubmed-meshheading:12762840-Cytoskeleton, pubmed-meshheading:12762840-Genes, Reporter, pubmed-meshheading:12762840-Genome, Human, pubmed-meshheading:12762840-Green Fluorescent Proteins, pubmed-meshheading:12762840-Humans, pubmed-meshheading:12762840-Liver Neoplasms, pubmed-meshheading:12762840-Luminescent Proteins, pubmed-meshheading:12762840-Microtubules, pubmed-meshheading:12762840-Mitochondria, pubmed-meshheading:12762840-Oligopeptides, pubmed-meshheading:12762840-Open Reading Frames, pubmed-meshheading:12762840-Protein Subunits, pubmed-meshheading:12762840-Recombinant Fusion Proteins, pubmed-meshheading:12762840-Repetitive Sequences, Amino Acid, pubmed-meshheading:12762840-Transfection, pubmed-meshheading:12762840-Tumor Cells, Cultured
pubmed:articleTitle
Novel complex integrating mitochondria and the microtubular cytoskeleton with chromosome remodeling and tumor suppressor RASSF1 deduced by in silico homology analysis, interaction cloning in yeast, and colocalization in cultured cells.
pubmed:affiliation
Center for Cancer Biology and Nutrition, Institute of Biosciences and Technology, Texas A&M University System Health Science Center, 2121 W. Holcombe Boulevard, Houston, Texas 77030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.