Linked Life Data

12761821

Source:http://linkedlifedata.com/resource/pubmed/id/12761821

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-5-22
pubmed:abstractText
Rasmussen's encephalitis is a childhood disease resulting in intractable seizures associated with hippocampal and neocortical inflammation. An autoantibody against the GluR3 subunit of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors is implicated in the pathophysiology of Rasmussen's encephalitis. AMPA receptors mediate excitatory neurotransmission in the brain and contain combinations of four subunits (GluR1-4). Although the distributions of GluR1, GluR2, and GluR4 are known in some detail, the cellular distribution of GluR3 in the mammalian brain remains to be described. We developed and characterized a GluR3-specific monoclonal antibody and quantified the cellular distribution of GluR3 in CA1 of the rat hippocampus. GluR3 immunoreactivity was detected in all pyramidal neurons and astrocytes and in most interneurons. We quantified the intensity of GluR3 immunoreactivity in interneuron subtypes defined by their calcium-binding protein content. GluR3 immunofluorescence, but not GluR1 or GluR2 immunofluorescence, was significantly elevated in somata of parvalbumin-containing interneurons compared to pyramidal somata. Strikingly, increased GluR3 immunofluorescence was not observed in calbindin- and calretinin-containing interneurons. Furthermore, 24% of parvalbumin-containing interneurons could be distinguished from surrounding neurons based on their intense GluR3 immunoreactivity. This subpopulation had significantly elevated GluR3 immunoreactivity compared to the rest of parvalbumin-containing interneurons. Electron microscopy revealed enriched GluR3 immunoreactivity in parvalbumin-containing perikarya at cytoplasmic and postsynaptic sites. Parvalbumin-containing interneurons, potent inhibitors of cortical pyramidal neurons, are vulnerable in the brains of epileptic patients. Our findings suggest that the somata of these interneurons are enriched in GluR3, which may render them vulnerable to pathological states such as epilepsy and Rasmussen's encephalitis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9967
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
462
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-28
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12761821-Animals, pubmed-meshheading:12761821-Autoantibodies, pubmed-meshheading:12761821-Calcium-Binding Protein, Vitamin D-Dependent, pubmed-meshheading:12761821-Encephalitis, pubmed-meshheading:12761821-Hippocampus, pubmed-meshheading:12761821-Immunohistochemistry, pubmed-meshheading:12761821-Interneurons, pubmed-meshheading:12761821-Male, pubmed-meshheading:12761821-Mice, pubmed-meshheading:12761821-Mice, Inbred BALB C, pubmed-meshheading:12761821-Microscopy, Electron, pubmed-meshheading:12761821-Neural Inhibition, pubmed-meshheading:12761821-Neural Pathways, pubmed-meshheading:12761821-Parvalbumins, pubmed-meshheading:12761821-Pyramidal Cells, pubmed-meshheading:12761821-Rats, pubmed-meshheading:12761821-Rats, Sprague-Dawley, pubmed-meshheading:12761821-Receptors, AMPA, pubmed-meshheading:12761821-Synapses, pubmed-meshheading:12761821-Up-Regulation
pubmed:year
2003
pubmed:articleTitle
Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus.
pubmed:affiliation
Kastor Neurobiology of Aging Laboratories, Mount Sinai School of Medicine, New York, New York 10029, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.