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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2003-5-21
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pubmed:abstractText |
Recently human cartilage gp-39 (HC gp-39) was identified as a candidate autoantigen in rheumatoid arthritis (RA). To further investigate the relevance of this Ag in RA, we have generated a set of five mAbs to a combination epitope of complexes of HC gp-39(263-275) and the RA-associated DR alpha beta 1*0401 HLA class II molecules. FACS studies revealed that these mAb recognize specific complexes on homozygous DR alpha beta 1*0401-positive B lymphoblastoid cells pulsed with HC gp-39(263-275). The best mAb, 12A, was further characterized using a set of irrelevant DR alpha beta 1*0401-binding peptides and truncated/elongated versions of HC gp-39(263-275) itself. The minimal epitope recognized in combination with DR alpha beta 1*0401 was HC gp-39(263-273). Peptides not encompassing HC gp-39(263-273) were not recognized. Three of five mAb were able to inhibit (up to 90%) the response of HC gp-39(263-275)-specific DR alpha beta 1*0401-restricted T cell hybridomas to peptide-pulsed APC or purified complexes. Using mAb 12A, we have been able to identify and localize dendritic cells that present DR alpha beta 1*0401/HC gp-39(263-275) complexes in synovial tissue of DR alpha beta 1*0401-positive RA patients, indicating local presentation of the HC gp-39(263-275) epitope in the inflamed target tissue by professional APC. These data support a role of HC gp-39 in the local autoimmune response that leads to chronic inflammation and joint destruction.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adipokines,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/CHI3L1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DRB1 Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
170
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5719-27
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:12759455-Adipokines,
pubmed-meshheading:12759455-Adult,
pubmed-meshheading:12759455-Aged,
pubmed-meshheading:12759455-Aged, 80 and over,
pubmed-meshheading:12759455-Animals,
pubmed-meshheading:12759455-Antibodies, Monoclonal,
pubmed-meshheading:12759455-Antibody Specificity,
pubmed-meshheading:12759455-Antigen Presentation,
pubmed-meshheading:12759455-Antigen-Presenting Cells,
pubmed-meshheading:12759455-Arthritis, Rheumatoid,
pubmed-meshheading:12759455-Autoantigens,
pubmed-meshheading:12759455-B-Lymphocyte Subsets,
pubmed-meshheading:12759455-Cell Line, Transformed,
pubmed-meshheading:12759455-Epitopes,
pubmed-meshheading:12759455-Female,
pubmed-meshheading:12759455-Glycoproteins,
pubmed-meshheading:12759455-HLA-DR Antigens,
pubmed-meshheading:12759455-HLA-DRB1 Chains,
pubmed-meshheading:12759455-Humans,
pubmed-meshheading:12759455-Hybridomas,
pubmed-meshheading:12759455-Immunosuppressive Agents,
pubmed-meshheading:12759455-Lectins,
pubmed-meshheading:12759455-Lymphocyte Activation,
pubmed-meshheading:12759455-Mice,
pubmed-meshheading:12759455-Mice, Inbred BALB C,
pubmed-meshheading:12759455-Middle Aged,
pubmed-meshheading:12759455-Peptide Fragments,
pubmed-meshheading:12759455-Synovial Membrane
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pubmed:year |
2003
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pubmed:articleTitle |
Localization of MHC class II/human cartilage glycoprotein-39 complexes in synovia of rheumatoid arthritis patients using complex-specific monoclonal antibodies.
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pubmed:affiliation |
Department of Pharmacology, N. V. Organon, Oss, The Netherlands. peter.steembakkers@organon.com
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pubmed:publicationType |
Journal Article
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