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rdf:type |
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lifeskim:mentions |
umls-concept:C0003069,
umls-concept:C0020964,
umls-concept:C0022564,
umls-concept:C0025936,
umls-concept:C0085424,
umls-concept:C0205227,
umls-concept:C0221912,
umls-concept:C0332120,
umls-concept:C0525037,
umls-concept:C1413292,
umls-concept:C1423526,
umls-concept:C1819451,
umls-concept:C2349975,
umls-concept:C2753500
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pubmed:issue |
11
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pubmed:dateCreated |
2003-5-21
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pubmed:abstractText |
p40, the common subunit of the proinflammatory cytokines IL-12 and IL-23, is produced by resident skin cells. Whereas the in vivo effects of IL-12 are well established, little is known about the role of IL-23 in cutaneous immune responses. In this study we show that p40 transgenic (TG) mice constitutively produce IL-23 (p19/p40), but not IL-12 (p35/p40), in basal keratinocytes by cosecretion of TG p40 with endogenous p19. Repeated injections of rIL-23 in littermate (LM) mice result in an inflammatory skin disease similar to that of p40 TG mice, confirming the proinflammatory activity of IL-23. Furthermore, IL-23 secretion by p40 TG keratinocytes induces elevated numbers of Langerhans cells (LC) with a marked up-regulation of costimulatory molecules, indicating advanced maturation of keratin 14 (K14)/p40 LC when compared with LM LC. At the functional level, freshly isolated K14/p40 LC greatly exceeded LC from LM animals in their capacity to stimulate allogeneic T cell proliferation. To assess whether IL-23 regulates cutaneous immune responses in vivo, we used an allogeneic skin transplantation model. Full thickness skin grafts from K14/p40 donors (H-2(q)) transplanted across a MHC class I and class II barrier onto BALB/c (H-2(d)) recipients were rejected in a significantly accelerated fashion (mean survival time: 8.8 days) when compared with skin grafts from non-TG LM (H-2(q)) (mean survival time: 10.7 days, p < 0.01). Based on these results we propose that IL-23-induced changes of LC may be an important mechanism in directing the outcome of cutaneous immune responses.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Il23a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12 Subunit p40,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-23,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-23 Subunit p19,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Keratin-14,
http://linkedlifedata.com/resource/pubmed/chemical/Keratins,
http://linkedlifedata.com/resource/pubmed/chemical/Krt1-14 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
170
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5438-44
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12759419-Animals,
pubmed-meshheading:12759419-Cell Count,
pubmed-meshheading:12759419-Cell Movement,
pubmed-meshheading:12759419-Cells, Cultured,
pubmed-meshheading:12759419-Female,
pubmed-meshheading:12759419-Graft Rejection,
pubmed-meshheading:12759419-Immunity, Cellular,
pubmed-meshheading:12759419-Immunophenotyping,
pubmed-meshheading:12759419-Inflammation,
pubmed-meshheading:12759419-Injections, Subcutaneous,
pubmed-meshheading:12759419-Interleukin-12,
pubmed-meshheading:12759419-Interleukin-12 Subunit p40,
pubmed-meshheading:12759419-Interleukin-23,
pubmed-meshheading:12759419-Interleukin-23 Subunit p19,
pubmed-meshheading:12759419-Interleukins,
pubmed-meshheading:12759419-Interphase,
pubmed-meshheading:12759419-Keratin-14,
pubmed-meshheading:12759419-Keratins,
pubmed-meshheading:12759419-Langerhans Cells,
pubmed-meshheading:12759419-Lymphocyte Activation,
pubmed-meshheading:12759419-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:12759419-Mice,
pubmed-meshheading:12759419-Mice, Inbred BALB C,
pubmed-meshheading:12759419-Mice, Transgenic,
pubmed-meshheading:12759419-Organ Culture Techniques,
pubmed-meshheading:12759419-Protein Subunits,
pubmed-meshheading:12759419-RNA, Messenger,
pubmed-meshheading:12759419-Skin,
pubmed-meshheading:12759419-Skin Diseases,
pubmed-meshheading:12759419-Skin Transplantation
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pubmed:year |
2003
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pubmed:articleTitle |
IL-23 production by cosecretion of endogenous p19 and transgenic p40 in keratin 14/p40 transgenic mice: evidence for enhanced cutaneous immunity.
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pubmed:affiliation |
Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases, University of Vienna Medical School, Vienna International Research Cooperation Center, Vienna, Austria. tamara.kopp@ahk-wien.ac.at
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pubmed:publicationType |
Journal Article
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