Linked Life Data

12759348

Source:http://linkedlifedata.com/resource/pubmed/id/12759348

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
31
pubmed:dateCreated
2003-7-28
pubmed:abstractText
The balance and cross-talk between natruretic and antinatruretic hormone receptors plays a critical role in the regulation of renal Na+ homeostasis, which is a major determinant of blood pressure. Dopamine and angiotensin II have antagonistic effects on renal Na+ and water excretion, which involves regulation of the Na+,K+-ATPase activity. Herein we demonstrate that angiotensin II (Ang II) stimulation of AT1 receptors in proximal tubule cells induces the recruitment of Na+,K+-ATPase molecules to the plasmalemma, in a process mediated by protein kinase Cbeta and interaction of the Na+,K+-ATPase with adaptor protein 1. Ang II stimulation led to phosphorylation of the alpha subunit Ser-11 and Ser-18 residues, and substitution of these amino acids with alanine residues completely abolished the Ang II-induced stimulation of Na+,K+-ATPase-mediated Rb+ transport. Thus, for Ang II-dependent stimulation of Na+,K+-ATPase activity, phosphorylation of these serine residues is essential and may constitute a triggering signal for recruitment of Na+,K+-ATPase molecules to the plasma membrane. When cells were treated simultaneously with saturating concentrations of dopamine and Ang II, either activation or inhibition of the Na+,K+-ATPase activity was produced dependent on the intracellular Na+ concentration, which was varied in a very narrow physiological range (9-19 mm). A small increase in intracellular Na+ concentrations induces the recruitment of D1 receptors to the plasma membrane and a reduction in plasma membrane AT1 receptors. Thus, one or more proteins may act as an intracellular Na+ concentration sensor and play a major regulatory role on the effect of hormones that regulate proximal tubule Na+ reabsorption.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
278
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28719-26
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12759348-Absorption, pubmed-meshheading:12759348-Angiotensin II, pubmed-meshheading:12759348-Animals, pubmed-meshheading:12759348-Cell Line, pubmed-meshheading:12759348-Cell Membrane, pubmed-meshheading:12759348-Dopamine, pubmed-meshheading:12759348-Epithelial Cells, pubmed-meshheading:12759348-Homeostasis, pubmed-meshheading:12759348-Kidney, pubmed-meshheading:12759348-Kidney Tubules, Proximal, pubmed-meshheading:12759348-Opossums, pubmed-meshheading:12759348-Phosphorylation, pubmed-meshheading:12759348-Protein Kinase C, pubmed-meshheading:12759348-Receptor, Angiotensin, Type 1, pubmed-meshheading:12759348-Receptors, Angiotensin, pubmed-meshheading:12759348-Receptors, Dopamine D1, pubmed-meshheading:12759348-Rubidium, pubmed-meshheading:12759348-Serine, pubmed-meshheading:12759348-Signal Transduction, pubmed-meshheading:12759348-Sodium, pubmed-meshheading:12759348-Sodium-Potassium-Exchanging ATPase, pubmed-meshheading:12759348-Tetradecanoylphorbol Acetate, pubmed-meshheading:12759348-Transfection
pubmed:year
2003
pubmed:articleTitle
Intracellular Na+ regulates dopamine and angiotensin II receptors availability at the plasma membrane and their cellular responses in renal epithelia.
pubmed:affiliation
College of Pharmacy, University of Houston, Houston, Texas 77204, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't