Linked Life Data

12759347

Source:http://linkedlifedata.com/resource/pubmed/id/12759347

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:12759347rdf:typepubmed:Citationlld:pubmed
pubmed-article:12759347lifeskim:mentionsumls-concept:C0007600lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C0024501lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C0008051lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C0012929lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C0205245lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C0965025lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C1514562lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C1883221lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C0883208lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C1883204lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C1880389lld:lifeskim
pubmed-article:12759347lifeskim:mentionsumls-concept:C1707513lld:lifeskim
pubmed-article:12759347pubmed:issue33lld:pubmed
pubmed-article:12759347pubmed:dateCreated2003-8-11lld:pubmed
pubmed-article:12759347pubmed:databankReferencehttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:databankReferencehttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:abstractTextNuclear and mitochondrial (mt) forms of chicken mt transcription factor A (c-TFAM) generated by alternative splicing of a gene (c-tfam) were cloned. c-tfam mapped at 6q1.1-q1.2 has similar exon/intron organization as mouse tfam except that the first exons encoding the nuclear and the mt form-specific sequences were positioned oppositely. When cDNA encoding the nuclear form was transiently expressed in chicken lymphoma DT40 cells after tagging at the C terminus with c-Myc, the product was localized into nucleus, whereas the only endogenous mt form of DT40 cells was immunostained exclusively within mitochondria. c-TFAM is most similar to Xenopus (xl-) TFAM in having extended C-terminal regions in addition to two high mobility group (HMG) boxes, a linker region between them, and a C-terminal tail, also found in human and mouse TFAM. Similarities between c- and xl-TFAM are higher in linker and C-terminal regions than in HMG boxes. Disruption of both tfam alleles in DT40 cells prevented proliferation. The tfam+/tfam- cells showed a 50 and 40-60% reduction of mtDNA and its transcripts, respectively. Expression of exogenous wild type c-tfam cDNA in the tfam+/tfam- cells increased mtDNA up to 4-fold in a dose-dependent manner, whereas its transcripts increased only marginally. A deletion mutant lacking the first HMG box lost this activity, whereas only marginal reduction of the activity was observed in a deletion mutant at the second HMG box. Despite the essential role of the C-terminal tail in mtDNA transcription demonstrated in vitro, deletion of c-TFAM at this region reduced the activity of maintenance of the mtDNA level only by 50%. A series of deletion mutant at the tail region suggested stimulatory and suppressive sequences in this region for the maintenance of mtDNA level.lld:pubmed
pubmed-article:12759347pubmed:languageenglld:pubmed
pubmed-article:12759347pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:citationSubsetIMlld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12759347pubmed:statusMEDLINElld:pubmed
pubmed-article:12759347pubmed:monthAuglld:pubmed
pubmed-article:12759347pubmed:issn0021-9258lld:pubmed
pubmed-article:12759347pubmed:authorpubmed-author:MatsudaYoichi...lld:pubmed
pubmed-article:12759347pubmed:authorpubmed-author:IshiiShojiSlld:pubmed
pubmed-article:12759347pubmed:authorpubmed-author:KitagawaYasuo...lld:pubmed
pubmed-article:12759347pubmed:authorpubmed-author:InagakiHideto...lld:pubmed
pubmed-article:12759347pubmed:authorpubmed-author:MatsumuraKiyo...lld:pubmed
pubmed-article:12759347pubmed:authorpubmed-author:SuzukiTomohir...lld:pubmed
pubmed-article:12759347pubmed:authorpubmed-author:MatsushimaYui...lld:pubmed
pubmed-article:12759347pubmed:authorpubmed-author:BeckKonradKlld:pubmed
pubmed-article:12759347pubmed:issnTypePrintlld:pubmed
pubmed-article:12759347pubmed:day15lld:pubmed
pubmed-article:12759347pubmed:volume278lld:pubmed
pubmed-article:12759347pubmed:ownerNLMlld:pubmed
pubmed-article:12759347pubmed:authorsCompleteYlld:pubmed
pubmed-article:12759347pubmed:pagination31149-58lld:pubmed
pubmed-article:12759347pubmed:dateRevised2006-11-15lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:meshHeadingpubmed-meshheading:12759347...lld:pubmed
pubmed-article:12759347pubmed:year2003lld:pubmed
pubmed-article:12759347pubmed:articleTitleFunctional domains of chicken mitochondrial transcription factor A for the maintenance of mitochondrial DNA copy number in lymphoma cell line DT40.lld:pubmed
pubmed-article:12759347pubmed:affiliationGraduate Courses for Regulation of Biological Signals, Nagoya University, Nagoya-shi, Japan.lld:pubmed
pubmed-article:12759347pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12759347pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
entrez-gene:373888entrezgene:pubmedpubmed-article:12759347lld:entrezgene
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12759347lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12759347lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12759347lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12759347lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12759347lld:pubmed