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rdf:type |
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lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0008051,
umls-concept:C0012929,
umls-concept:C0024501,
umls-concept:C0205245,
umls-concept:C0883208,
umls-concept:C0965025,
umls-concept:C1514562,
umls-concept:C1707513,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
33
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pubmed:dateCreated |
2003-8-11
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pubmed:databankReference |
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pubmed:abstractText |
Nuclear and mitochondrial (mt) forms of chicken mt transcription factor A (c-TFAM) generated by alternative splicing of a gene (c-tfam) were cloned. c-tfam mapped at 6q1.1-q1.2 has similar exon/intron organization as mouse tfam except that the first exons encoding the nuclear and the mt form-specific sequences were positioned oppositely. When cDNA encoding the nuclear form was transiently expressed in chicken lymphoma DT40 cells after tagging at the C terminus with c-Myc, the product was localized into nucleus, whereas the only endogenous mt form of DT40 cells was immunostained exclusively within mitochondria. c-TFAM is most similar to Xenopus (xl-) TFAM in having extended C-terminal regions in addition to two high mobility group (HMG) boxes, a linker region between them, and a C-terminal tail, also found in human and mouse TFAM. Similarities between c- and xl-TFAM are higher in linker and C-terminal regions than in HMG boxes. Disruption of both tfam alleles in DT40 cells prevented proliferation. The tfam+/tfam- cells showed a 50 and 40-60% reduction of mtDNA and its transcripts, respectively. Expression of exogenous wild type c-tfam cDNA in the tfam+/tfam- cells increased mtDNA up to 4-fold in a dose-dependent manner, whereas its transcripts increased only marginally. A deletion mutant lacking the first HMG box lost this activity, whereas only marginal reduction of the activity was observed in a deletion mutant at the second HMG box. Despite the essential role of the C-terminal tail in mtDNA transcription demonstrated in vitro, deletion of c-TFAM at this region reduced the activity of maintenance of the mtDNA level only by 50%. A series of deletion mutant at the tail region suggested stimulatory and suppressive sequences in this region for the maintenance of mtDNA level.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Mitochondrial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/High Mobility Group Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitochondrial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TFAM protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/TFAM protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tfam protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/XL-MTTFA protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/mitochondrial transcription factor A
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31149-58
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12759347-Amino Acid Sequence,
pubmed-meshheading:12759347-Animals,
pubmed-meshheading:12759347-Chickens,
pubmed-meshheading:12759347-Cloning, Molecular,
pubmed-meshheading:12759347-DNA, Complementary,
pubmed-meshheading:12759347-DNA, Mitochondrial,
pubmed-meshheading:12759347-DNA-Binding Proteins,
pubmed-meshheading:12759347-Gene Dosage,
pubmed-meshheading:12759347-Gene Expression,
pubmed-meshheading:12759347-High Mobility Group Proteins,
pubmed-meshheading:12759347-Humans,
pubmed-meshheading:12759347-Lymphoma,
pubmed-meshheading:12759347-Mice,
pubmed-meshheading:12759347-Mitochondrial Proteins,
pubmed-meshheading:12759347-Molecular Sequence Data,
pubmed-meshheading:12759347-Mutagenesis,
pubmed-meshheading:12759347-Nuclear Proteins,
pubmed-meshheading:12759347-Protein Structure, Tertiary,
pubmed-meshheading:12759347-Sequence Homology, Amino Acid,
pubmed-meshheading:12759347-Trans-Activators,
pubmed-meshheading:12759347-Transcription Factors,
pubmed-meshheading:12759347-Tumor Cells, Cultured,
pubmed-meshheading:12759347-Xenopus Proteins,
pubmed-meshheading:12759347-Xenopus laevis
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pubmed:year |
2003
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pubmed:articleTitle |
Functional domains of chicken mitochondrial transcription factor A for the maintenance of mitochondrial DNA copy number in lymphoma cell line DT40.
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pubmed:affiliation |
Graduate Courses for Regulation of Biological Signals, Nagoya University, Nagoya-shi, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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