Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-5-20
pubmed:abstractText
Chromosome segregation during mitosis requires kinetochores, specialized organelles that mediate chromosome attachment to spindle microtubules. We have shown previously that in budding yeast, Plc1p (phosphoinositide-specific phospholipase C) localizes to centromeric loci, associates with the kinetochore proteins Ndc10p and Cep3p, and affects the function of kinetochores. Deletion of PLC1 results in nocodazole sensitivity, mitotic delay, and a higher frequency of chromosome loss. We report here that despite the nocodazole sensitivity of plc1Delta cells, Plc1p is not required for the spindle checkpoint. However, plc1Delta cells require a functional BUB1/BUB3-dependent spindle checkpoint for viability. PLC1 displays strong genetic interactions with genes encoding components of the inner kinetochore, including NDC10, SKP1, MIF2, CEP1, CEP3, and CTF13. Furthermore, plc1Delta cells display alterations in chromatin structure in the core centromere. Chromatin immunoprecipitation experiments indicate that Plc1p localizes to centromeric loci independently of microtubules, and accumulates at the centromeres during G(2)/M stage of cell cycle. These results are consistent with the view that Plc1p affects kinetochore function, possibly by modulating the structure of centromeric chromatin.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1617-4615
pubmed:author
pubmed:issnType
Print
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-70
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12756538-Cell Nucleus, pubmed-meshheading:12756538-Cell Survival, pubmed-meshheading:12756538-Chromatin, pubmed-meshheading:12756538-DNA-Binding Proteins, pubmed-meshheading:12756538-Dose-Response Relationship, Drug, pubmed-meshheading:12756538-Gene Deletion, pubmed-meshheading:12756538-Genotype, pubmed-meshheading:12756538-Kinetochores, pubmed-meshheading:12756538-Microscopy, Fluorescence, pubmed-meshheading:12756538-Mitosis, pubmed-meshheading:12756538-Mutation, pubmed-meshheading:12756538-Nuclear Proteins, pubmed-meshheading:12756538-Plasmids, pubmed-meshheading:12756538-Precipitin Tests, pubmed-meshheading:12756538-Saccharomyces cerevisiae, pubmed-meshheading:12756538-Saccharomyces cerevisiae Proteins, pubmed-meshheading:12756538-Saccharomycetales, pubmed-meshheading:12756538-Temperature, pubmed-meshheading:12756538-Time Factors, pubmed-meshheading:12756538-Type C Phospholipases
pubmed:year
2003
pubmed:articleTitle
Genetic evidence for a role of phospholipase C at the budding yeast kinetochore.
pubmed:affiliation
Department of Biological Sciences, St. John's University, 8000 Utopia Parkway, Jamaicam New York, NY 11439, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't