Linked Life Data

12753489

Source:http://linkedlifedata.com/resource/pubmed/id/12753489

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-5-19
pubmed:abstractText
Endurance training elicits profound adaptations of skeletal muscle, including increased expression of several proteins. The 5'-AMP activated protein kinase (AMPK) may be one of these, considering the fact that acute exercise increases AMPK activity. Eight young (26 +/- 1 year) lean, healthy males endurance trained one leg (while the other leg remained resting) on an ergometer bicycle for 30 min/day for four weeks (workload corresponding to approximately 70% of maximal oxygen uptake). Muscle biopsies were obtained approximately 18 h after the previous training session. On day eight GLUT4 protein expression was 36% higher in trained (T) compared with untrained (UT) (P < 0.05), but no further increase was seen at day 14 and 30 despite continuously increasing absolute workloads. Expression of AMPKalpha2 and actin did not change with training. In contrast, expression of AMPKalpha1 was 27% higher in T vs. UT muscle (P < 0.05) (measured only on day 30). Conclusions: GLUT4 protein expression increases substantially after seven days of endurance training with no further increase with prolonged training at progressively increasing workloads. AMPKalpha1 and alpha2 behave differently in their expression in response to endurance training. AMPKalpha1 protein content is increased after one month of training, while no change in AMPKalpha2 and actin expression was detected over the time course of the training period.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0905-7188
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-74
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Time course of GLUT4 and AMPK protein expression in human skeletal muscle during one month of physical training.
pubmed:affiliation
Copenhagen Muscle Research Center, Department of Medical Physiology, The Panum Institute, University of Copenhagen, Denmark.
pubmed:publicationType
Journal Article, Clinical Trial, Comparative Study, Research Support, Non-U.S. Gov't