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rdf:type |
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lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0017262,
umls-concept:C0036098,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0040203,
umls-concept:C0085295,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0282509,
umls-concept:C0591833,
umls-concept:C1413235,
umls-concept:C1519477,
umls-concept:C1555707,
umls-concept:C1705851,
umls-concept:C2752151,
umls-concept:C2828366,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
2003-5-19
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pubmed:abstractText |
Tick saliva contains immunosuppressive factors allowing this blood-feeding ectoparasite to remain on hosts and enhancing pathogen transmission. In this study, we examined the modulation of mitogen-induced activation of naive murine splenocytes by the saliva and salivary gland extract (SGE) of I. ricinus ticks. We found that saliva-specific factors reduced IL-10 production by both concanavalin A (ConA) and lipopolysaccharide (LPS) stimulated splenocytes. The LPS-induced IL-10 production is 10 times more sensitive to SGE than the ConA-induced IL-10 production. Flow cytometric analysis determined that SGE particularly inhibited B (B220+) cell IL-10 production in mitogen-stimulated splenocyte preparations. Moreover, SGE reduced the early activation marker CD69 expression on ConA-activated T cells and also on B cells in presence of ConA or LPS. Annexin V and Via-probe staining demonstrated that SGE did not increase cell death in activated splenocytes and slightly decreased apoptosis in B lymphocytes. By employing assays with isolated B cells, we further showed that SGE had a direct effect on B cells and inhibited LPS-induced B cell proliferation. Taken together, our results indicate that salivary immunomodulators induce hyporesponsiveness to mitogen in both T and B cells, and that a direct B-cell inhibitory activity is present in tick saliva.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD69 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0141-9838
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27-37
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12753435-Animals,
pubmed-meshheading:12753435-Antigens, CD,
pubmed-meshheading:12753435-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:12753435-B-Lymphocytes,
pubmed-meshheading:12753435-Cell Extracts,
pubmed-meshheading:12753435-Cells, Cultured,
pubmed-meshheading:12753435-Concanavalin A,
pubmed-meshheading:12753435-Dose-Response Relationship, Drug,
pubmed-meshheading:12753435-Female,
pubmed-meshheading:12753435-Humans,
pubmed-meshheading:12753435-Interleukin-10,
pubmed-meshheading:12753435-Ixodes,
pubmed-meshheading:12753435-Lectins, C-Type,
pubmed-meshheading:12753435-Lipopolysaccharides,
pubmed-meshheading:12753435-Lymphocyte Activation,
pubmed-meshheading:12753435-Mice,
pubmed-meshheading:12753435-Mice, Inbred BALB C,
pubmed-meshheading:12753435-Mice, Inbred C3H,
pubmed-meshheading:12753435-Salivary Glands,
pubmed-meshheading:12753435-Spleen,
pubmed-meshheading:12753435-T-Lymphocytes,
pubmed-meshheading:12753435-Time Factors
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pubmed:year |
2003
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pubmed:articleTitle |
Ixodes ricinus tick salivary gland extract inhibits IL-10 secretion and CD69 expression by mitogen-stimulated murine splenocytes and induces hyporesponsiveness in B lymphocytes.
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pubmed:affiliation |
School of Biological Science (Zoology), University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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