Linked Life Data

12752628

Source:http://linkedlifedata.com/resource/pubmed/id/12752628

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-5-19
pubmed:abstractText
Angiogenesis is an important prognostic factor in infiltrating ductal carcinoma (IDC). Vascular endothelial growth factor (VEGF) stimulates angiogenesis in vivo. VEGF expression has been correlated with high vascularity in IDC. However, little is known about the prognostic significance of microvessel density (MVD) and its correlation with the expression of VEGF in infiltrating lobular carcinoma (ILC). We analyzed tumor samples from 51 patients with primary classic ILC to determine the relationship between tumoral MVD and VEGF expression. Cases of pleomorphic lobular carcinoma and tubulolobular carcinoma were excluded. Five-micron thick sections obtained from formalin-fixed, paraffin-embedded tissue blocks were immunostained with antibodies to factor VIII-related antigen (Dako, Carpenteria, CA) and VEGF (Calbiochem, Boston, MA). The former was used for MVD analysis. The vessel counts from the three most vascular fields (x200 magnification) were recorded and the highest of the vessel counts of the three fields was designated as the MVD. The intensity of VEGF staining and the proportion of cells staining were scored. Both the vessel counts and the scoring of VEGF staining were evaluated by two independent pathologists. The Student's t-test and Wilcoxon rank sum test were used to compare mean MVD and VEGF scores according to various clinical and pathologic features. All significance tests were two-sided with an alpha-level of 0.05. There was good correlation between the MVD of each observer (correlation coefficient 0.775, p < 0.001). There was no correlation of MVD or VEGF score with the size or stage of the tumor. In addition, the MVD or VEGF score was not significantly different between axillary lymph node-positive cases and node-negative cases, between patients with recurrence and those without, and between patients who survived and those who died of disease. There was, however, a weak negative correlation between the MVD and VEGF expression (Spearman correlation coefficient -0.08). Neither MVD or VEGF immunoscore were associated with tumor recurrence or vital status in patients with ILC. The absence of a statistically significant positive correlation between MVD and VEGF expression suggests that other factors may play a more important role in the angiogenesis of ILC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1075-122X
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
200-7
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:12752628-Adult, pubmed-meshheading:12752628-Aged, pubmed-meshheading:12752628-Aged, 80 and over, pubmed-meshheading:12752628-Breast Neoplasms, pubmed-meshheading:12752628-Carcinoma, Ductal, Breast, pubmed-meshheading:12752628-Carcinoma, Lobular, pubmed-meshheading:12752628-Endothelial Growth Factors, pubmed-meshheading:12752628-Female, pubmed-meshheading:12752628-Humans, pubmed-meshheading:12752628-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:12752628-Lymphokines, pubmed-meshheading:12752628-Middle Aged, pubmed-meshheading:12752628-Neoplasm Staging, pubmed-meshheading:12752628-Neovascularization, Pathologic, pubmed-meshheading:12752628-Retrospective Studies, pubmed-meshheading:12752628-Vascular Endothelial Growth Factor A, pubmed-meshheading:12752628-Vascular Endothelial Growth Factors
pubmed:articleTitle
Microvessel density and vascular endothelial growth factor expression in infiltrating lobular mammary carcinoma.
pubmed:affiliation
Department of Pathology and Biostatistics Unit, University of Alabama at Birmingham, Birmingham, Alabama 35249, USA. dchhieng@path.uab.edu
pubmed:publicationType
Journal Article