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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-5-16
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pubmed:abstractText |
The human DF3/MUC1 transmembrane protein is aberrantly expressed in multiple myeloma cells and other B cell malignancies. The regulation of MUC1 in B cells and its potential function as a signaling molecule are unknown. The present results demonstrate that interleukin-7 (IL-7) stimulates MUC1 expression in multiple myeloma cells. The results also demonstrate the IL-7 induces binding of MUC1 to the Lyn tyrosine kinase. The MUC1 C-terminal subunit binds directly to Lyn through interactions with the Lyn SH3 and SH2 domains. Activation of Lyn in response to IL-7 stimulation results in increased tyrosine phosphorylation of the MUC1 C-terminal subunit. In vitro and in vivo studies show that Lyn phosphorylates MUC1, at least in large part, on a YEKV site in the MUC1 cytoplasmic tail. The functional significance of the MUC1-Lyn interaction is supported by the demonstration that Lyn-mediated phosphorylation of MUC1 on YEKV induces binding of MUC1 and the beta-catenin signaling protein. In concert with these results, IL-7 treatment is associated with binding of MUC1 to beta-catenin and targeting of the MUC1-beta-catenin complex to the nucleus. These findings indicate that IL-7 regulates MUC1 expression and function in multiple myeloma cells.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/Mucin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/lyn protein-tyrosine kinase,
http://linkedlifedata.com/resource/pubmed/chemical/src-Family Kinases
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pubmed:status |
MEDLINE
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pubmed:issn |
1538-4047
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
187-93
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:12750561-Amino Acid Sequence,
pubmed-meshheading:12750561-Cytoskeletal Proteins,
pubmed-meshheading:12750561-DNA Primers,
pubmed-meshheading:12750561-Fluorescent Antibody Technique,
pubmed-meshheading:12750561-Humans,
pubmed-meshheading:12750561-Immunoblotting,
pubmed-meshheading:12750561-Interleukin-7,
pubmed-meshheading:12750561-Molecular Sequence Data,
pubmed-meshheading:12750561-Mucin-1,
pubmed-meshheading:12750561-Multiple Myeloma,
pubmed-meshheading:12750561-Phosphorylation,
pubmed-meshheading:12750561-Polymerase Chain Reaction,
pubmed-meshheading:12750561-Precipitin Tests,
pubmed-meshheading:12750561-Protein Binding,
pubmed-meshheading:12750561-Recombinant Proteins,
pubmed-meshheading:12750561-Signal Transduction,
pubmed-meshheading:12750561-Trans-Activators,
pubmed-meshheading:12750561-Transfection,
pubmed-meshheading:12750561-Tumor Cells, Cultured,
pubmed-meshheading:12750561-Tyrosine,
pubmed-meshheading:12750561-beta Catenin,
pubmed-meshheading:12750561-src-Family Kinases
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pubmed:articleTitle |
DF3/MUC1 signaling in multiple myeloma cells is regulated by interleukin-7.
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pubmed:affiliation |
Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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