Linked Life Data

12750294

Source:http://linkedlifedata.com/resource/pubmed/id/12750294

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2003-5-16
pubmed:abstractText
The cytoplasmic and nuclear redistribution of beta-catenin and the de novo expression of vimentin are frequently involved in the epithelial-to-mesenchymal transition associated with increased invasive/migratory properties of epithelial cells. Because beta-catenin can act as a coactivator of transcription through its binding to the T-cell factor (TCF)/lymphoid enhancer factor 1 transcription factor family, we have explored the possibility that beta-catenin/TCF could directly transactivate vimentin. We first compared vimentin expression in relation with the localization of beta-catenin in eight breast cancer cell lines displaying various degrees of invasiveness and in a model of cell migration using human mammary MCF10A cells. We could thus show a cytoplasmic and/or nuclear distribution of beta-catenin in invasive/migratory cells expressing vimentin, but not in noninvasive/stationary vimentin-negative cell lines. In addition, the human vimentin promoter was found to be up-regulated by beta-catenin and TCF-4 cotransfection. Varying with the cellular background, a diminution of this up-regulation was observed when the putative beta-catenin/TCF binding site of the vimentin promoter was mutated. Our results therefore demonstrate that the vimentin promoter is a target of the beta-catenin/TCF pathway and strongly suggest an implication of this regulation in epithelial cell migration/invasion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2658-64
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:12750294-Breast Neoplasms, pubmed-meshheading:12750294-Cell Nucleus, pubmed-meshheading:12750294-Cytoplasm, pubmed-meshheading:12750294-Cytoskeletal Proteins, pubmed-meshheading:12750294-DNA-Binding Proteins, pubmed-meshheading:12750294-Gene Expression Regulation, Neoplastic, pubmed-meshheading:12750294-Humans, pubmed-meshheading:12750294-Promoter Regions, Genetic, pubmed-meshheading:12750294-TCF Transcription Factors, pubmed-meshheading:12750294-Trans-Activators, pubmed-meshheading:12750294-Transcription Factor 7-Like 2 Protein, pubmed-meshheading:12750294-Transcription Factors, pubmed-meshheading:12750294-Transcriptional Activation, pubmed-meshheading:12750294-Transfection, pubmed-meshheading:12750294-Tumor Cells, Cultured, pubmed-meshheading:12750294-Vimentin, pubmed-meshheading:12750294-beta Catenin
pubmed:year
2003
pubmed:articleTitle
Transactivation of vimentin by beta-catenin in human breast cancer cells.
pubmed:affiliation
Laboratory of Tumor and Developmental Biology, University of Liège, CHU Sart-Tilman, B23, B-4000 Liège, Belgium. cgilles@ulg.ac.be
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't