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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2003-5-15
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pubmed:abstractText |
T-cell depletion is an essential step in reducing the risk of graft-versus-host disease (GVHD) in patients with inherited metabolic storage diseases (IMSD) undergoing hematopoietic stem cell transplantation. This goal can be achieved either by selective removal of T cells or by positive selection of CD34+ cells. Large-scale preparations of purified CD34+ cells from bone marrow products have not been extensively described. We report our results with bone marrow CD34+ cell enrichment using the CliniMACS system in eight children with IMSD. The median recovery of positively selected CD34+ cells was 46.2% with a purity of 97.5%, and a residual T cell content of 0.04 x 10(6). A median of 5.5 x 10(6)/kg of CD34+ cells was infused. All patients engrafted at a median time of 12 days and none of the patients developed GVHD. This method is technically feasible and can be successfully used to transplant children with IMSD.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0268-3369
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pubmed:author |
pubmed-author:BalduzziAA,
pubmed-author:BertagnolioBB,
pubmed-author:BiondiAA,
pubmed-author:BonanomiSS,
pubmed-author:CortiPP,
pubmed-author:DassaEE,
pubmed-author:GaipaGG,
pubmed-author:LongoniDD,
pubmed-author:MaseraGG,
pubmed-author:PariniRR,
pubmed-author:PerseghinPP,
pubmed-author:PetersCC,
pubmed-author:RovelliAA,
pubmed-author:UderzoCC,
pubmed-author:UzielGG,
pubmed-author:VenturaVV
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
857-60
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12748661-Antigens, CD34,
pubmed-meshheading:12748661-B-Lymphocytes,
pubmed-meshheading:12748661-Child,
pubmed-meshheading:12748661-Child, Preschool,
pubmed-meshheading:12748661-Female,
pubmed-meshheading:12748661-Graft vs Host Disease,
pubmed-meshheading:12748661-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:12748661-Humans,
pubmed-meshheading:12748661-Immunomagnetic Separation,
pubmed-meshheading:12748661-Infant,
pubmed-meshheading:12748661-Leukodystrophy, Globoid Cell,
pubmed-meshheading:12748661-Lymphocyte Depletion,
pubmed-meshheading:12748661-Male,
pubmed-meshheading:12748661-Mucopolysaccharidosis I,
pubmed-meshheading:12748661-T-Lymphocytes,
pubmed-meshheading:12748661-Transplantation, Homologous,
pubmed-meshheading:12748661-Treatment Outcome
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pubmed:year |
2003
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pubmed:articleTitle |
Allogeneic bone marrow stem cell transplantation following CD34+ immunomagnetic enrichment in patients with inherited metabolic storage diseases.
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pubmed:affiliation |
Clinica Pediatrica, Università di Milano-Bicocca, Ospedale San Gerardo, Monza, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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